63333

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 354579..355105 Replicon chromosome
Accession NZ_CP015749
Organism Pectobacterium parmentieri strain RNS 08-42-1A

Toxin (Protein)


Gene name ccdB Uniprot ID A0A8B3FHC0
Locus tag A8F97_RS01525 Protein ID WP_033071873.1
Coordinates 354579..354884 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID C6C380
Locus tag A8F97_RS01530 Protein ID WP_015855076.1
Coordinates 354887..355105 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8F97_RS22870 350670..350891 - 222 Protein_296 integrase -
A8F97_RS01510 351927..353258 + 1332 WP_033071876.1 hypothetical protein -
A8F97_RS01515 353260..353838 + 579 WP_033071875.1 hypothetical protein -
A8F97_RS01520 353936..354473 - 538 Protein_299 exonuclease SbcC -
A8F97_RS01525 354579..354884 - 306 WP_033071873.1 type II toxin-antitoxin system toxin CcdB Toxin
A8F97_RS01530 354887..355105 - 219 WP_015855076.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A8F97_RS01535 355164..355907 - 744 WP_033071872.1 hypothetical protein -
A8F97_RS24310 356049..356219 + 171 WP_154665134.1 hypothetical protein -
A8F97_RS01545 356811..357152 - 342 WP_033071870.1 hypothetical protein -
A8F97_RS01550 357338..357532 + 195 WP_033071869.1 DUF3950 domain-containing protein -
A8F97_RS01555 357638..358897 - 1260 WP_069704166.1 integrase arm-type DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 351114..359064 7950


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11773.64 Da        Isoelectric Point: 4.4978

>T63333 WP_033071873.1 NZ_CP015749:c354884-354579 [Pectobacterium parmentieri]
MQFIVYQYRRASRYKMFVDVQSDIVETPKRRMVIPLIEAHNLSEKVNNTLFPLIRIDGEDYRLMTTELSSVPVEVMGEVI
ADLGDYADEIKDAINLMFWGI

Download         Length: 306 bp

>T63333 NZ_CP015749:c354884-354579 [Pectobacterium parmentieri]
ATGCAATTCATTGTTTATCAATACAGACGCGCCAGCCGCTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCCAAGCGGCGCATGGTCATCCCGCTTATCGAAGCACATAACCTGTCTGAGAAAGTGAATAATACGCTGTTTCCTCTGA
TCCGCATCGACGGCGAAGATTATCGGCTGATGACGACTGAACTATCGAGCGTACCTGTTGAAGTCATGGGTGAAGTGATT
GCGGATCTTGGCGACTACGCGGATGAGATCAAAGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8178.13 Da        Isoelectric Point: 7.0135

>AT63333 WP_015855076.1 NZ_CP015749:c355105-354887 [Pectobacterium parmentieri]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Download         Length: 219 bp

>AT63333 NZ_CP015749:c355105-354887 [Pectobacterium parmentieri]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTACCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
AGTGAATGACGCCATTGGCAAAGAAGCCCGCCGCATCAAGGCTGAGGAGTGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCGCAGAATGGCTCGTTTGCGGATGAAAACAGGAACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3FHC0

Antitoxin

Source ID Structure
AlphaFold DB A0A250BG46

References