63321

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4923619..4924261 Replicon chromosome
Accession NZ_CP015727
Organism Bacillus cereus strain A1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag DA68_RS24665 Protein ID WP_000635965.1
Coordinates 4923911..4924261 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag DA68_RS24660 Protein ID WP_000004570.1
Coordinates 4923619..4923906 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DA68_RS24635 4918936..4919898 + 963 WP_000961161.1 UV DNA damage repair endonuclease UvsE -
DA68_RS24640 4919891..4920463 - 573 WP_025966355.1 rhomboid family intramembrane serine protease -
DA68_RS24645 4920557..4920916 + 360 WP_000583417.1 holo-ACP synthase -
DA68_RS24650 4921073..4922023 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
DA68_RS24655 4922141..4923310 + 1170 WP_000390617.1 alanine racemase -
DA68_RS24660 4923619..4923906 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
DA68_RS24665 4923911..4924261 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DA68_RS24670 4924329..4926497 + 2169 WP_000426236.1 RNA-binding transcriptional accessory protein -
DA68_RS29985 4926555..4926671 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
DA68_RS24675 4926867..4927325 + 459 WP_000344242.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T63321 WP_000635965.1 NZ_CP015727:4923911-4924261 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T63321 NZ_CP015727:4923911-4924261 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT63321 WP_000004570.1 NZ_CP015727:4923619-4923906 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT63321 NZ_CP015727:4923619-4923906 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References