Detailed information of TA system TA063320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 1639210..1640188
• Replicon: chromosome
• Accession: NZ_CP015727
• Organism: Bacillus cereus strain A1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: DA68_RS08405
• Protein ID: WP_000624979.1
• Coordinates: 1639210..1639947 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: DA68_RS08410
• Protein ID: WP_000237818.1
• Coordinates: 1640060..1640188 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DA68_RS08385	1634850..1635635	+	786	WP_029960449.1	class I SAM-dependent methyltransferase	-
DA68_RS08390	1635793..1637478	-	1686	WP_029960448.1	alpha-keto acid decarboxylase family protein	-
DA68_RS08395	1637586..1638068	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
DA68_RS08400	1638235..1638972	+	738	WP_000594151.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
DA68_RS08405	1639210..1639947	+	738	WP_000624979.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
DA68_RS08410	1640060..1640188	+	129	WP_000237818.1	hypothetical protein	Antitoxin
DA68_RS08415	1640261..1640437	+	177	WP_000852627.1	stage II sporulation protein SB	-
DA68_RS08420	1640457..1640846	-	390	WP_000713864.1	YxeA family protein	-
DA68_RS08425	1641058..1642527	+	1470	WP_000287533.1	beta-Ala-His dipeptidase	-
DA68_RS08430	1642772..1643581	+	810	WP_000664542.1	C39 family peptidase	-
DA68_RS08435	1643607..1644209	+	603	WP_000517260.1	hypothetical protein	-
DA68_RS08440	1644450..1644701	-	252	WP_000827560.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28311.04 Da
• Isoelectric Point: 8.2998

>T63320 WP_000624979.1 NZ_CP015727:1639210-1639947 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHMEQLNEQKAVQVENVGVIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T63320 NZ_CP015727:1639210-1639947 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
AGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTAGATATTGCATTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCCATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATACGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATGGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGGTTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT63320 WP_000237818.1 NZ_CP015727:1640060-1640188 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT63320 NZ_CP015727:1640060-1640188 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5