Detailed information of TA system TA063319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 5449024..5449658
• Replicon: chromosome
• Accession: NZ_CP015726
• Organism: Streptomyces sp. RTd22

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A7B19_RS22500
• Protein ID: WP_063730354.1
• Coordinates: 5449236..5449658 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: A7B19_RS22495
• Protein ID: WP_063730346.1
• Coordinates: 5449024..5449239 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7B19_RS22485	5446878..5448133	+	1256	Protein_4565	helix-turn-helix transcriptional regulator	-
A7B19_RS22490	5448152..5448628	+	477	WP_063730335.1	hypothetical protein	-
A7B19_RS22495	5449024..5449239	+	216	WP_063730346.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
A7B19_RS22500	5449236..5449658	+	423	WP_063730354.1	PIN domain nuclease	Toxin
A7B19_RS22505	5449931..5450656	-	726	WP_063730357.1	hypothetical protein	-
A7B19_RS22510	5450752..5451705	-	954	WP_064273790.1	DMT family transporter	-
A7B19_RS22515	5451756..5452360	-	605	Protein_4571	TetR/AcrR family transcriptional regulator	-
A7B19_RS22520	5452401..5452928	-	528	WP_063730363.1	hypothetical protein	-
A7B19_RS22525	5453077..5454576	+	1500	WP_079151953.1	transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-46)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15421.35 Da
• Isoelectric Point: 5.1653

>T63319 WP_063730354.1 NZ_CP015726:5449236-5449658 [Streptomyces sp. RTd22]
VNAAQFLIDTSALARFMRGDAEQYGWDQAAAAGLIATCPITELEFFYSARSAADRAHGIEDMRLMFGRVLVDDRAYDRAW
QVQEALTKQGKHRSAGPVDLVVAATAELQGLTLLHRDHDFECIAAVTGQALQWYGPEHGK

Nucleotide

• Length: 423 bp

>T63319 NZ_CP015726:5449236-5449658 [Streptomyces sp. RTd22]
GTGAACGCGGCCCAGTTCCTGATCGACACCAGCGCGCTCGCGCGGTTCATGCGCGGGGACGCGGAGCAGTACGGCTGGGA
CCAGGCCGCCGCGGCTGGACTCATCGCGACCTGCCCCATTACCGAGCTTGAGTTCTTCTACAGCGCACGGTCGGCCGCTG
ACCGGGCACACGGCATCGAGGACATGCGGCTGATGTTCGGCAGGGTTCTCGTCGACGACCGCGCCTACGACCGCGCCTGG
CAGGTCCAGGAAGCCCTCACCAAGCAGGGGAAACACCGAAGTGCCGGACCAGTCGATCTGGTCGTCGCCGCGACCGCTGA
GCTGCAGGGGCTCACCCTGCTCCATCGCGATCACGACTTCGAGTGCATCGCCGCAGTGACCGGGCAAGCCCTCCAGTGGT
ATGGCCCGGAGCACGGCAAGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7862.91 Da
• Isoelectric Point: 8.6595

>AT63319 WP_063730346.1 NZ_CP015726:5449024-5449239 [Streptomyces sp. RTd22]
MSRTVIDLDDDLVADVAKALGTNTKKETVNTALREVLESRRRALALARLRAAAGDGAFDLELFENKGNYRR

Nucleotide

• Length: 216 bp

>AT63319 NZ_CP015726:5449024-5449239 [Streptomyces sp. RTd22]
ATGAGCCGGACGGTTATCGACCTGGACGACGACCTGGTCGCGGACGTGGCGAAGGCCCTCGGGACGAACACCAAGAAGGA
GACCGTCAACACCGCTCTTCGCGAGGTGTTGGAGAGCCGGCGTCGCGCCCTGGCTCTCGCCCGGCTGCGGGCCGCGGCGG
GCGACGGCGCATTTGATCTGGAGCTGTTCGAGAACAAGGGGAACTACCGCCGGTGA