Detailed information of TA system TA063290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 67314..67843
• Replicon: chromosome
• Accession: NZ_CP015645
• Organism: Staphylococcus aureus strain 08-02119

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A7P66_RS00370
• Protein ID: WP_000621175.1
• Coordinates: 67481..67843 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: A7P66_RS00365
• Protein ID: WP_000948331.1
• Coordinates: 67314..67484 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7P66_RS00335	62350..62910	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
A7P66_RS00340	63119..63598	+	480	WP_001287079.1	hypothetical protein	-
A7P66_RS00345	63591..65174	+	1584	WP_001294620.1	PH domain-containing protein	-
A7P66_RS00350	65161..65652	+	492	WP_001205912.1	PH domain-containing protein	-
A7P66_RS00355	65656..66015	+	360	WP_000581202.1	holo-ACP synthase	-
A7P66_RS00360	66081..67229	+	1149	WP_000130547.1	alanine racemase	-
A7P66_RS00365	67314..67484	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
A7P66_RS00370	67481..67843	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A7P66_RS00380	68192..69193	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
A7P66_RS00385	69312..69638	+	327	WP_001052491.1	anti-sigma factor antagonist	-
A7P66_RS00390	69640..70119	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
A7P66_RS00395	70094..70864	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T63290 WP_000621175.1 NZ_CP015645:67481-67843 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T63290 NZ_CP015645:67481-67843 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT63290 WP_000948331.1 NZ_CP015645:67314-67484 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT63290 NZ_CP015645:67314-67484 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1