Detailed information of TA system TA063286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 983292..983806
• Replicon: chromosome
• Accession: NZ_CP015642
• Organism: Staphylococcus simulans strain MR1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A7X73_RS04465
• Protein ID: WP_031262808.1
• Coordinates: 983456..983806 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A2T4QNU3
• Locus tag: A7X73_RS04460
• Protein ID: WP_023016097.1
• Coordinates: 983292..983459 (+)
• Length: 56 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7X73_RS04435	979182..979646	+	465	WP_105967036.1	PH domain-containing protein	-
A7X73_RS04440	979639..981132	+	1494	WP_105967037.1	PH domain-containing protein	-
A7X73_RS04445	981125..981622	+	498	WP_023016100.1	PH domain-containing protein	-
A7X73_RS04450	981624..981977	+	354	WP_031262810.1	holo-ACP synthase	-
A7X73_RS04455	982057..983211	+	1155	WP_105967038.1	alanine racemase	-
A7X73_RS04460	983292..983459	+	168	WP_023016097.1	hypothetical protein	Antitoxin
A7X73_RS04465	983456..983806	+	351	WP_031262808.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A7X73_RS04470	984098..985099	+	1002	WP_106420981.1	PP2C family protein-serine/threonine phosphatase	-
A7X73_RS04475	985175..985501	+	327	WP_105967039.1	anti-sigma factor antagonist	-
A7X73_RS04480	985503..985982	+	480	WP_023016096.1	anti-sigma B factor RsbW	-
A7X73_RS04485	985957..986727	+	771	WP_002481747.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12976.09 Da
• Isoelectric Point: 10.0474

>T63286 WP_031262808.1 NZ_CP015642:983456-983806 [Staphylococcus simulans]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIGISLGLTHKN

Nucleotide

• Length: 351 bp

>T63286 NZ_CP015642:983456-983806 [Staphylococcus simulans]
ATGATGAGGAGAGGCGATGTTTATTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGACCTGTTGT
AATTATACAAAATGACACTGGCAATAAGTACAGTCCGACGGTAATTGTAGCCGCGATTACTGGGCGTATTAATAAGGCGA
AAATTCCAACACATGTTGAAATTGAAAAGAGTAAATACAAATTAGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTTGATAAAAAACGTTTGAAAGAGAAATTGACTTATCTTTCAGATGAGAAGATGAAAGAAATTGATAACGCGATTGG
AATTAGTTTAGGCTTGACACATAAAAATTAG

Antitoxin

Protein

• Length: 56 a.a.
• Molecular weight: 6257.78 Da
• Isoelectric Point: 3.6836

>AT63286 WP_023016097.1 NZ_CP015642:983292-983459 [Staphylococcus simulans]
MSTFNQNRSYSLEQSLKEGYSQMADLNLSLATEAFSVECEACDCNESYLTFDKDE

Nucleotide

• Length: 168 bp

>AT63286 NZ_CP015642:983292-983459 [Staphylococcus simulans]
ATGTCAACTTTTAATCAGAATAGAAGCTATAGTTTGGAACAGTCATTAAAAGAAGGCTATTCACAAATGGCTGATTTGAA
TCTCTCCCTAGCAACGGAGGCATTTTCAGTGGAATGTGAAGCCTGTGATTGCAATGAATCTTATCTTACATTTGATAAGG
ATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2T4QNU3