Detailed information of TA system TA063284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 12913..13505
• Replicon: plasmid unnamed
• Accession: NZ_CP015640
• Organism: Pseudomonas lurida strain L228

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A263NKW4
• Locus tag: A7318_RS28065
• Protein ID: WP_069078795.1
• Coordinates: 12913..13212 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: A7318_RS28070
• Protein ID: WP_069078796.1
• Coordinates: 13209..13505 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7318_RS28045	9363..10370	-	1008	WP_069078791.1	ParB/RepB/Spo0J family partition protein	-
A7318_RS28050	10460..11593	-	1134	WP_069078792.1	ParA family protein	-
A7318_RS28055	11677..11910	-	234	WP_069078793.1	hypothetical protein	-
A7318_RS28060	11990..12400	-	411	WP_069078794.1	hypothetical protein	-
A7318_RS28065	12913..13212	+	300	WP_069078795.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A7318_RS28070	13209..13505	+	297	WP_069078796.1	putative addiction module antidote protein	Antitoxin
A7318_RS28075	13618..14076	-	459	WP_069078797.1	hypothetical protein	-
A7318_RS28080	14225..14776	+	552	WP_069078798.1	GNAT family N-acetyltransferase	-
A7318_RS28085	14773..15750	+	978	WP_069078799.1	tyrosine-type recombinase/integrase	-
A7318_RS28090	15793..16884	-	1092	WP_069078800.1	hypothetical protein	-
A7318_RS29865	17131..17301	-	171	WP_167356514.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..77900	77900

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (51-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11184.90 Da
• Isoelectric Point: 10.4000

>T63284 WP_069078795.1 NZ_CP015640:12913-13212 [Pseudomonas lurida]
MKTIKQTATYMTWERKLKDQRAKAAIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFAQRGEEFVLLLCGGDKS
TQSRDIEAAKKLAIQWRQE

Nucleotide

• Length: 300 bp

>T63284 NZ_CP015640:12913-13212 [Pseudomonas lurida]
ATGAAGACGATAAAACAAACAGCGACCTACATGACGTGGGAACGCAAACTCAAGGACCAGCGCGCCAAGGCCGCTATCGC
CGCGCGGATCTTCCGCTTGGCGAACGGACTACCAGGTGATGTGTCACCAGTAGGGCAGGGCGTCAGTGAGTTGCGAATCC
ATTACGGGCCGGGCTACCGGGTCTATTTCGCACAGCGCGGTGAAGAGTTTGTTTTGTTGTTGTGCGGCGGTGACAAGTCC
ACACAAAGCCGCGATATAGAAGCGGCCAAAAAACTCGCGATCCAGTGGAGGCAAGAATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10494.83 Da
• Isoelectric Point: 4.7628

>AT63284 WP_069078796.1 NZ_CP015640:13209-13505 [Pseudomonas lurida]
MNEAIYDYDPAEALNGPEAIAIFMADAFETNDADHIAQALGVVARAKGMGELAKETGLSRQQLYRSFSKNGNPTLKTLLA
VTRALGVDMTARPHAETA

Nucleotide

• Length: 297 bp

>AT63284 NZ_CP015640:13209-13505 [Pseudomonas lurida]
ATGAACGAAGCAATTTACGACTACGATCCAGCAGAAGCCCTGAACGGCCCTGAGGCCATCGCCATCTTTATGGCAGACGC
CTTTGAGACCAACGATGCTGATCACATCGCCCAGGCCCTGGGCGTCGTTGCACGCGCTAAAGGGATGGGTGAACTGGCCA
AGGAAACCGGTCTGTCCCGGCAGCAGCTTTACCGGTCATTCAGCAAGAACGGTAACCCCACACTGAAAACCCTGCTGGCC
GTGACGCGCGCCCTGGGTGTCGACATGACTGCACGGCCTCACGCTGAAACTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A263NKW4