Detailed information of TA system TA063283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4804392..4805434
• Replicon: chromosome
• Accession: NZ_CP015639
• Organism: Pseudomonas lurida strain L228

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A855QJK0
• Locus tag: A7318_RS21815
• Protein ID: WP_034109273.1
• Coordinates: 4804859..4805434 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: A7318_RS21810
• Protein ID: WP_069078199.1
• Coordinates: 4804392..4804862 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7318_RS21785	4799421..4800773	+	1353	WP_034109278.1	sensor histidine kinase	-
A7318_RS21790	4801158..4801730	+	573	WP_069078195.1	hypothetical protein	-
A7318_RS28980	4801622..4802023	-	402	Protein_4364	hypothetical protein	-
A7318_RS21795	4802132..4802755	-	624	WP_069078196.1	DUF3916 domain-containing protein	-
A7318_RS21800	4802780..4803037	-	258	WP_069078197.1	colicin transporter	-
A7318_RS21805	4803045..4804199	-	1155	WP_069078198.1	S-type pyocin domain-containing protein	-
A7318_RS21810	4804392..4804862	+	471	WP_069078199.1	helix-turn-helix domain-containing protein	Antitoxin
A7318_RS21815	4804859..4805434	+	576	WP_034109273.1	PIN domain-containing protein	Toxin
A7318_RS21820	4805553..4805924	-	372	WP_034109272.1	PH domain-containing protein	-
A7318_RS21825	4806082..4806717	+	636	WP_069078200.1	LysE family translocator	-
A7318_RS21830	4806724..4807053	-	330	WP_069078201.1	hypothetical protein	-
A7318_RS21835	4807272..4808168	+	897	WP_069078202.1	hypothetical protein	-
A7318_RS21840	4808199..4808483	+	285	WP_069078203.1	hypothetical protein	-
A7318_RS21845	4808644..4809024	-	381	WP_034109267.1	cupin domain-containing protein	-
A7318_RS21850	4809644..4809943	-	300	WP_034109382.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4802780..4823685	20905

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21538.85 Da
• Isoelectric Point: 5.0339

>T63283 WP_034109273.1 NZ_CP015639:4804859-4805434 [Pseudomonas lurida]
MRCSPFTAVYDACVLYPAALRDLLMWLGLSGLYRARWTSQIHEEWKRNVLKNRPDLTAEHVNCTSALMDKAIPDALVTGY
EDLCSSLDLPDPDDRHVLAAAIRSKAEVIVTFNLKDFPTQILSAYDIEPMHPDDFISDLWDLDQAAVLGAAQKQRRALKR
PPMEAGSYLEMLMRQGLAQTSKLLGPYEVML

Nucleotide

• Length: 576 bp

>T63283 NZ_CP015639:4804859-4805434 [Pseudomonas lurida]
ATGAGGTGTTCGCCTTTTACCGCTGTATATGATGCTTGTGTTCTCTACCCGGCGGCGCTTAGGGATTTGCTGATGTGGCT
TGGCCTGTCAGGGCTGTATCGAGCGAGATGGACTAGCCAGATTCATGAGGAGTGGAAGCGCAACGTACTCAAGAACCGAC
CCGATCTCACTGCTGAGCATGTGAATTGCACGTCAGCGCTGATGGACAAGGCTATACCTGACGCTTTGGTTACTGGATAT
GAGGATCTGTGTTCGAGCTTGGATCTGCCCGATCCGGATGATCGTCACGTACTGGCGGCCGCAATTCGGAGTAAAGCTGA
GGTTATTGTGACCTTCAATTTGAAAGACTTCCCGACGCAGATCCTGTCTGCTTATGACATTGAGCCGATGCATCCGGATG
ACTTTATTTCGGATCTATGGGATCTGGATCAGGCGGCGGTGCTGGGGGCTGCGCAAAAGCAGAGGCGGGCGTTGAAGCGG
CCTCCGATGGAGGCGGGAAGTTATCTTGAGATGTTGATGCGACAAGGGCTGGCACAGACAAGTAAGCTGCTTGGACCTTA
TGAGGTCATGCTATAA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17455.09 Da
• Isoelectric Point: 5.6324

>AT63283 WP_069078199.1 NZ_CP015639:4804392-4804862 [Pseudomonas lurida]
MITNHLPKTILPLEKEVEAAVQGQRELASLLSTKFETQRIDIFDKEDKPHTLVLPTSALRLLVDILGELAMGNAVKVVPV
HAELTSQEAADLLNVSRPHLVKMLEEGAIPFTKTGRHRRIRFSDLMAFKQRRDEESQEAMEALANQAQELGMGYDG

Nucleotide

• Length: 471 bp

>AT63283 NZ_CP015639:4804392-4804862 [Pseudomonas lurida]
ATGATCACCAACCACCTTCCCAAAACGATCCTTCCGCTGGAAAAGGAAGTCGAAGCCGCCGTACAAGGCCAGCGCGAATT
GGCGTCCTTGCTATCGACCAAATTTGAAACCCAGCGCATCGACATCTTTGACAAAGAAGACAAACCCCACACCCTCGTAC
TCCCAACCTCAGCACTGCGCCTGTTGGTGGATATCCTGGGCGAGTTGGCTATGGGCAATGCCGTCAAGGTAGTCCCGGTG
CATGCCGAACTCACCTCGCAGGAGGCCGCTGACCTGCTTAACGTTTCACGGCCACACCTGGTCAAGATGCTGGAGGAAGG
CGCTATTCCCTTTACAAAAACCGGTCGGCATCGGCGAATCCGGTTTTCGGATTTGATGGCGTTCAAGCAGCGGCGAGATG
AGGAAAGCCAGGAAGCAATGGAAGCGCTGGCGAATCAGGCCCAGGAGCTGGGAATGGGGTATGACGGATGA

Structures

Toxin

• AlphaFold DB: A0A855QJK0