Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/upstrm_HI1419-dnstrm_HI1420
Location 667762..668355 Replicon chromosome
Accession NZ_CP015637
Organism Pseudomonas fluorescens strain L321

Toxin (Protein)


Gene name relE Uniprot ID A0A855JZ99
Locus tag A7317_RS02840 Protein ID WP_024073151.1
Coordinates 668053..668355 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A855JY32
Locus tag A7317_RS02835 Protein ID WP_024073150.1
Coordinates 667762..668049 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7317_RS02815 664165..666234 - 2070 WP_069075166.1 TonB-dependent copper receptor -
A7317_RS02820 666327..666728 - 402 WP_024073147.1 DUF2946 domain-containing protein -
A7317_RS02825 666739..667221 - 483 WP_069075167.1 copper chaperone PCu(A)C -
A7317_RS02830 667269..667664 - 396 WP_024073149.1 DUF2946 domain-containing protein -
A7317_RS02835 667762..668049 - 288 WP_024073150.1 putative addiction module antidote protein Antitoxin
A7317_RS02840 668053..668355 - 303 WP_024073151.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A7317_RS02845 668873..669127 + 255 WP_069075168.1 hypothetical protein -
A7317_RS02850 669171..669494 - 324 WP_024073153.1 transcriptional regulator -
A7317_RS02855 669500..669832 - 333 WP_024073154.1 addiction module protein -
A7317_RS02860 669889..670614 - 726 WP_024073155.1 cobalt-precorrin-6A reductase -
A7317_RS02865 670611..671816 - 1206 WP_024073156.1 bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase -
A7317_RS02870 671939..673201 + 1263 WP_155766425.1 precorrin-3B synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-95)

Antitoxin

Uncharacterized protein HI1420

(4-93)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11717.47 Da        Isoelectric Point: 10.1888

>T63277 WP_024073151.1 NZ_CP015637:c668355-668053 [Pseudomonas fluorescens]
MIQFKKSKHFLEWLDSLRSKPARARVLARLDHAQWGNFGDCEALGNGVFEMRIHYGPGYRVYFTRRGEVVYLLLIGGDKS
TQKRDIQRAKQIAEDFEKKE

Download         Length: 303 bp

>T63277 NZ_CP015637:c668355-668053 [Pseudomonas fluorescens]
ATGATTCAGTTTAAGAAGTCAAAACACTTTTTAGAATGGCTTGATTCGTTACGCAGTAAACCCGCGCGAGCACGCGTACT
GGCAAGGTTGGATCACGCGCAATGGGGCAACTTTGGAGACTGCGAAGCACTCGGAAATGGCGTTTTTGAAATGCGCATCC
ACTATGGCCCTGGCTACAGGGTTTATTTCACTCGCAGAGGCGAGGTGGTTTACCTGCTGTTGATCGGAGGCGACAAATCA
ACGCAGAAACGTGACATTCAGCGCGCTAAACAAATTGCAGAAGACTTTGAAAAAAAGGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10391.78 Da        Isoelectric Point: 4.4791

>AT63277 WP_024073150.1 NZ_CP015637:c668049-667762 [Pseudomonas fluorescens]
MNEFTDFDVAEHLKNPEDMAEYLEACFEEDTGDGLLIRSALNNIARAQGMTQVARDAGLGRESLYKALSSNGNPEFATIM
KVMKALGLKLHATAI

Download         Length: 288 bp

>AT63277 NZ_CP015637:c668049-667762 [Pseudomonas fluorescens]
ATGAATGAATTCACTGACTTCGACGTGGCGGAGCACCTAAAAAACCCCGAGGATATGGCCGAATATCTTGAAGCGTGCTT
CGAAGAAGATACGGGTGACGGTCTGCTGATCCGTTCAGCGCTAAACAACATCGCCCGCGCCCAAGGCATGACCCAAGTAG
CCCGCGATGCCGGCCTCGGAAGAGAAAGTCTATACAAAGCCCTCTCCAGCAATGGCAATCCCGAGTTCGCGACTATCATG
AAAGTCATGAAGGCTTTGGGCCTCAAGCTTCACGCTACAGCGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A855JZ99

Antitoxin

Source ID Structure
AlphaFold DB A0A855JY32

References