Detailed information of TA system TA063276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 733424..733948
• Replicon: chromosome
• Accession: NZ_CP015626
• Organism: Staphylococcus pseudintermedius strain 063228

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A6M57_RS03240
• Protein ID: WP_065520421.1
• Coordinates: 733595..733948 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A166MM16
• Locus tag: A6M57_RS03235
• Protein ID: WP_014613378.1
• Coordinates: 733424..733594 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6M57_RS03210 (A6M57_3410)	729313..729786	+	474	WP_014613373.1	PH domain-containing protein	-
A6M57_RS03215 (A6M57_3415)	729779..731308	+	1530	WP_014613374.1	PH domain-containing protein	-
A6M57_RS03220 (A6M57_3420)	731292..731810	+	519	WP_014613375.1	PH domain-containing protein	-
A6M57_RS03225 (A6M57_3425)	731807..732157	+	351	WP_014613376.1	holo-ACP synthase	-
A6M57_RS03230 (A6M57_3430)	732191..733339	+	1149	WP_065520767.1	alanine racemase	-
A6M57_RS03235 (A6M57_3435)	733424..733594	+	171	WP_014613378.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
A6M57_RS03240 (A6M57_3440)	733595..733948	+	354	WP_065520421.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A6M57_RS03245 (A6M57_3445)	734005..735010	+	1006	Protein_651	PP2C family protein-serine/threonine phosphatase	-
A6M57_RS03250 (A6M57_3450)	735089..735415	+	327	WP_014613381.1	anti-sigma factor antagonist	-
A6M57_RS03255 (A6M57_3455)	735415..735894	+	480	WP_031253870.1	anti-sigma B factor RsbW	-
A6M57_RS03260 (A6M57_3460)	735869..736639	+	771	WP_014613383.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13158.41 Da
• Isoelectric Point: 10.4439

>T63276 WP_065520421.1 NZ_CP015626:733595-733948 [Staphylococcus pseudintermedius]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAVITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEKKMKEVNNAIGISLGLHMNHHK

Nucleotide

• Length: 354 bp

>T63276 NZ_CP015626:733595-733948 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATCCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGTAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6396.04 Da
• Isoelectric Point: 3.9930

>AT63276 WP_014613378.1 NZ_CP015626:733424-733594 [Staphylococcus pseudintermedius]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYPVECEACDCNESHLTTDYKED

Nucleotide

• Length: 171 bp

>AT63276 NZ_CP015626:733424-733594 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A166MM16