Detailed information of TA system TA063271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 4685..5273
• Replicon: plasmid p4AsACE
• Accession: NZ_CP015619
• Organism: Acinetobacter schindleri strain ACE

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: AsACE_RS15830
• Protein ID: WP_039890878.1
• Coordinates: 4685..4972 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: AsACE_RS15835
• Protein ID: WP_076755249.1
• Coordinates: 4959..5273 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AsACE_RS15785	85..618	+	534	WP_076755239.1	plasmid replication DNA-binding protein	-
AsACE_RS15790	798..1121	-	324	WP_076755242.1	hypothetical protein	-
AsACE_RS15795	1227..1649	+	423	WP_157759225.1	hypothetical protein	-
AsACE_RS15800	1646..1972	+	327	WP_005407416.1	hypothetical protein	-
AsACE_RS15805	2455..2649	-	195	WP_005237343.1	hypothetical protein	-
AsACE_RS15810	2675..3286	-	612	WP_076755244.1	recombinase family protein	-
AsACE_RS15815	3559..3903	+	345	WP_076755246.1	QacE family quaternary ammonium compound efflux SMR transporter	-
AsACE_RS15820	3904..4461	+	558	WP_076755247.1	TetR/AcrR family transcriptional regulator	-
AsACE_RS15830	4685..4972	+	288	WP_039890878.1	BrnT family toxin	Toxin
AsACE_RS15835	4959..5273	+	315	WP_076755249.1	BrnA antitoxin family protein	Antitoxin
AsACE_RS15840	5339..5998	-	660	WP_081409112.1	protein phosphatase 2C domain-containing protein	-
AsACE_RS15845	6056..7363	-	1308	WP_076755253.1	serine/threonine protein kinase	-
AsACE_RS16275	7486..7662	-	177	WP_157759227.1	hypothetical protein	-
AsACE_RS15850	7659..8030	-	372	WP_000504220.1	hypothetical protein	-
AsACE_RS15865	9295..10218	+	924	WP_076755255.1	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10218	10218

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11263.65 Da
• Isoelectric Point: 5.2216

>T63271 WP_039890878.1 NZ_CP015619:4685-4972 [Acinetobacter schindleri]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T63271 NZ_CP015619:4685-4972 [Acinetobacter schindleri]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAGCATGATGTCTCTTTCGAAACCGCAAG
CCTTGTTTTTGAAGATCCTCTGAGGATATCAATCCAAGACAGACATACCGATGGCGAAGAACGTTGGCAGACCATTGGAA
AAGTGAAAGGCGTACTAATGCTCTTAGTAGCTCATACCATCTTTGATGAAGATGACTGTGAAATCATAAGAATTATTAGT
GCAAGACAGGTAACTAAAGCAGAGCGAGATAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12280.01 Da
• Isoelectric Point: 9.7969

>AT63271 WP_076755249.1 NZ_CP015619:4959-5273 [Acinetobacter schindleri]
MSMVRYSRKELNKKFSDKQDAEIERLLAKGKVPDDQLDLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT63271 NZ_CP015619:4959-5273 [Acinetobacter schindleri]
ATGAGCATGGTTAGATACTCACGCAAAGAACTGAATAAAAAATTCAGCGACAAGCAAGATGCTGAAATTGAACGCTTGCT
TGCTAAGGGAAAGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGACTGGAGTAATGCTGTACGCC
ACAATCAATTCTATCGTCCAGTTAAGCAACAAACATCTATTCGTTTAGATGCTGACGTACTTGCATGGTTCAAAGCACAA
GGTAAAGGTTATCAGACACGCATGAATGAAATTTTGCGGGATGCAATGCTAAAAGAATTGAAAAATCATCAGTAA