Detailed information of TA system TA063270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 54021..54609
• Replicon: plasmid p1AsACE
• Accession: NZ_CP015616
• Organism: Acinetobacter schindleri strain ACE

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: V2UW36
• Locus tag: AsACE_RS14795
• Protein ID: WP_004884492.1
• Coordinates: 54322..54609 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: AsACE_RS14790
• Protein ID: WP_023274838.1
• Coordinates: 54021..54335 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AsACE_RS14770	49603..50388	+	786	WP_076755016.1	acetoin reductase	-
AsACE_RS14775	50410..51468	+	1059	WP_004281883.1	2,3-butanediol dehydrogenase	-
AsACE_RS14780	51811..53136	+	1326	WP_081409111.1	ISNCY family transposase	-
AsACE_RS14785	53299..53928	-	630	WP_076755020.1	hypothetical protein	-
AsACE_RS14790	54021..54335	-	315	WP_023274838.1	BrnA antitoxin family protein	Antitoxin
AsACE_RS14795	54322..54609	-	288	WP_004884492.1	BrnT family toxin	Toxin
AsACE_RS14800	54940..55929	-	990	WP_005093385.1	copper resistance protein B	-
AsACE_RS14805	55916..57658	-	1743	WP_076755023.1	multicopper oxidase domain-containing protein	-
AsACE_RS14815	58387..58572	+	186	Protein_45	helix-turn-helix domain-containing protein	-
AsACE_RS14820	58626..59330	-	705	WP_004897650.1	IS6-like element IS1006 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..179461	179461
	-	flank	IS/Tn	-	-	51799..53136	1337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11277.63 Da
• Isoelectric Point: 5.2134

>T63270 WP_004884492.1 NZ_CP015616:c54609-54322 [Acinetobacter schindleri]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHG

Nucleotide

• Length: 288 bp

>T63270 NZ_CP015616:c54609-54322 [Acinetobacter schindleri]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAGCACGATGTCTCTTTCGAAACAGCAAG
CCTTGTTTTTGATGATCCATTAAGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAA
AAGTAAAAGGCGTACTAATGCTCTTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTCACCAAAGCGGAGCGAGATAGATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12298.98 Da
• Isoelectric Point: 9.4860

>AT63270 WP_023274838.1 NZ_CP015616:c54335-54021 [Acinetobacter schindleri]
MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAVRHNRFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT63270 NZ_CP015616:c54335-54021 [Acinetobacter schindleri]
ATGAGCATGGTTAGATACTCACGCAAAGAACTGAATGAAAAATTCAGCGACAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAGGGAACGGTTCCTGACGATCAGCTAGATCTCTCAGATATGCCTGAAATTACCGACTGGAGCAATGCTGTACGCC
ATAATCGATTCTATCGCCCCGTGAAGCAACAGACTTCCATTCGTTTAGATGCTGATGTACTTGCATGGTTTAAAGCTCAA
GGCAAAGGCTATCAAACACGAATGAATGAAATTTTGCGGGATGCTATGCTCAAAGAATTAAAAAATCATCAGTAA

Structures

Toxin

• AlphaFold DB: A0A3F3L125