Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3312819..3313455 Replicon chromosome
Accession NZ_CP015611
Organism Bacillus safensis strain U17-1

Toxin (Protein)


Gene name mazF Uniprot ID A0A0P7G713
Locus tag BSA171_RS17430 Protein ID WP_024425388.1
Coordinates 3312819..3313169 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag BSA171_RS17435 Protein ID WP_003214273.1
Coordinates 3313174..3313455 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSA171_RS17390 3307850..3308449 - 600 WP_041089211.1 SpoIIE family protein phosphatase -
BSA171_RS17395 3308449..3309237 - 789 WP_024427378.1 RNA polymerase sigma factor SigB -
BSA171_RS17400 3309203..3309691 - 489 WP_075610925.1 anti-sigma B factor RsbW -
BSA171_RS17405 3309688..3310017 - 330 WP_017358393.1 anti-sigma factor antagonist -
BSA171_RS17410 3310077..3311084 - 1008 WP_039176714.1 PP2C family protein-serine/threonine phosphatase -
BSA171_RS17415 3311095..3311496 - 402 WP_024425390.1 anti-sigma regulatory factor -
BSA171_RS17420 3311499..3311867 - 369 WP_003214235.1 RsbT antagonist protein RsbS -
BSA171_RS17425 3311872..3312702 - 831 WP_075610924.1 RsbT co-antagonist protein RsbRA -
BSA171_RS17430 3312819..3313169 - 351 WP_024425388.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BSA171_RS17435 3313174..3313455 - 282 WP_003214273.1 hypothetical protein Antitoxin
BSA171_RS17440 3313750..3314931 - 1182 WP_075610923.1 alanine racemase -
BSA171_RS17445 3315072..3316088 - 1017 WP_087975235.1 outer membrane lipoprotein carrier protein LolA -
BSA171_RS17450 3316249..3316614 - 366 WP_075610922.1 holo-ACP synthase -
BSA171_RS17455 3316709..3317314 + 606 WP_041089200.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8891

>T63241 WP_024425388.1 NZ_CP015611:c3313169-3312819 [Bacillus safensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T63241 NZ_CP015611:c3313169-3312819 [Bacillus safensis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCAGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTGATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTCTTGGAGCAGATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT63241 WP_003214273.1 NZ_CP015611:c3313455-3313174 [Bacillus safensis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT63241 NZ_CP015611:c3313455-3313174 [Bacillus safensis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTACCGGAGGCTTTGATGTCTGAAATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P7G713

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References