Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2009973..2010609 Replicon chromosome
Accession NZ_CP015607
Organism Bacillus safensis strain U14-5

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag BSA145_RS10445 Protein ID WP_003214169.1
Coordinates 2010259..2010609 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A267X601
Locus tag BSA145_RS10440 Protein ID WP_012008995.1
Coordinates 2009973..2010254 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSA145_RS10420 2006114..2006719 - 606 WP_041107457.1 rhomboid family intramembrane serine protease -
BSA145_RS10425 2006814..2007179 + 366 WP_075622395.1 holo-ACP synthase -
BSA145_RS10430 2007340..2008356 + 1017 WP_155762173.1 outer membrane lipoprotein carrier protein LolA -
BSA145_RS10435 2008498..2009679 + 1182 WP_075622397.1 alanine racemase -
BSA145_RS10440 2009973..2010254 + 282 WP_012008995.1 hypothetical protein Antitoxin
BSA145_RS10445 2010259..2010609 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BSA145_RS10450 2010725..2011555 + 831 WP_075622398.1 RsbT co-antagonist protein RsbRA -
BSA145_RS10455 2011560..2011928 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
BSA145_RS10460 2011931..2012332 + 402 WP_024425390.1 anti-sigma regulatory factor -
BSA145_RS10465 2012343..2013350 + 1008 WP_041089208.1 PP2C family protein-serine/threonine phosphatase -
BSA145_RS10470 2013410..2013739 + 330 WP_075622399.1 anti-sigma factor antagonist -
BSA145_RS10475 2013736..2014224 + 489 WP_075622400.1 anti-sigma B factor RsbW -
BSA145_RS10480 2014190..2014978 + 789 WP_075622401.1 RNA polymerase sigma factor SigB -
BSA145_RS10485 2014978..2015577 + 600 WP_075622402.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T63239 WP_003214169.1 NZ_CP015607:2010259-2010609 [Bacillus safensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T63239 NZ_CP015607:2010259-2010609 [Bacillus safensis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATCAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10894.27 Da        Isoelectric Point: 5.2726

>AT63239 WP_012008995.1 NZ_CP015607:2009973-2010254 [Bacillus safensis]
MSESSATNEMKIRLPETLMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT63239 NZ_CP015607:2009973-2010254 [Bacillus safensis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGACTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAACC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAGTTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A267X601

References