Detailed information of TA system TA063238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 13275..13903
• Replicon: plasmid pP27494_2
• Accession: NZ_CP015602
• Organism: Pseudomonas antarctica strain PAMC 27494

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A172ZAC2
• Locus tag: A7J50_RS30120
• Protein ID: WP_064455150.1
• Coordinates: 13505..13903 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A172ZA00
• Locus tag: A7J50_RS30115
• Protein ID: WP_064455149.1
• Coordinates: 13275..13505 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7J50_RS30085	8723..9019	+	297	WP_064455144.1	hypothetical protein	-
A7J50_RS31255	9087..9326	+	240	WP_082896019.1	antitoxin	-
A7J50_RS30090	9319..9720	+	402	WP_064455169.1	tRNA(fMet)-specific endonuclease VapC	-
A7J50_RS30095	9736..10182	+	447	WP_064455145.1	hypothetical protein	-
A7J50_RS30100	10981..11523	+	543	WP_064455146.1	hypothetical protein	-
A7J50_RS30105	11526..12671	+	1146	WP_064455147.1	hypothetical protein	-
A7J50_RS30110	12681..13016	-	336	WP_064455148.1	FxLYD domain-containing protein	-
A7J50_RS30115	13275..13505	+	231	WP_064455149.1	antitoxin	Antitoxin
A7J50_RS30120	13505..13903	+	399	WP_064455150.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A7J50_RS31260	14155..14334	+	180	WP_082896020.1	CrpP family protein	-
A7J50_RS30125	14331..14543	+	213	WP_064455151.1	hypothetical protein	-
A7J50_RS30130	14540..14947	+	408	WP_156526342.1	hypothetical protein	-
A7J50_RS30135	15314..15565	+	252	WP_064455153.1	hypothetical protein	-
A7J50_RS30140	16443..17252	+	810	WP_064455170.1	DUF3560 domain-containing protein	-
A7J50_RS30145	17470..17730	+	261	WP_064455154.1	hypothetical protein	-
A7J50_RS30150	17727..18347	+	621	WP_064455155.1	hypothetical protein	-
A7J50_RS30155	18401..18724	+	324	WP_064455156.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..30116	30116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14711.95 Da
• Isoelectric Point: 7.4348

>T63238 WP_064455150.1 NZ_CP015602:13505-13903 [Pseudomonas antarctica]
MLKFMLDTNICIFTIKNKPEHVREVFKAHHGQLCISTVTLMELIYGAEKSSNPGRNLADVEGFAARLEVLKYDQDAAAHT
GQLRAELAKAGTPIGPYDHMIAGHARSQGLILVTNNLREFNRVPGLRVEDWV

Nucleotide

• Length: 399 bp

>T63238 NZ_CP015602:13505-13903 [Pseudomonas antarctica]
ATGCTCAAGTTCATGCTGGACACCAACATCTGCATCTTCACCATAAAGAACAAGCCAGAGCATGTGCGTGAGGTGTTCAA
AGCGCACCACGGCCAACTGTGCATCAGCACTGTGACCTTGATGGAACTCATCTACGGCGCGGAAAAATCCTCTAACCCAG
GGCGAAACCTGGCTGATGTGGAGGGCTTCGCAGCACGGCTTGAAGTCCTGAAATACGATCAGGATGCGGCAGCGCACACA
GGGCAACTGAGGGCAGAGCTTGCCAAAGCGGGTACGCCCATTGGTCCTTATGACCACATGATCGCTGGGCACGCTCGTTC
GCAAGGGTTGATTTTGGTGACCAATAATCTACGCGAATTTAACCGCGTGCCGGGTTTGCGCGTTGAGGATTGGGTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8439.35 Da
• Isoelectric Point: 4.2683

>AT63238 WP_064455149.1 NZ_CP015602:13275-13505 [Pseudomonas antarctica]
MEQGSVFLSNRSQAVRLPKAAALPDDVKRVDVIAIGRTRILTPAGESWDSWFDGEGVTADFMADREQPADQERESF

Nucleotide

• Length: 231 bp

>AT63238 NZ_CP015602:13275-13505 [Pseudomonas antarctica]
ATGGAACAAGGTTCTGTATTTCTAAGTAATCGTAGCCAGGCTGTGCGCTTACCCAAGGCCGCTGCGCTGCCCGACGATGT
TAAGCGTGTGGATGTGATCGCCATAGGTAGAACGCGAATCCTCACGCCTGCTGGGGAGTCCTGGGATAGCTGGTTTGATG
GTGAGGGTGTGACGGCTGATTTTATGGCTGATCGTGAGCAACCCGCCGATCAAGAGCGGGAATCGTTCTGA

Structures

Toxin

• AlphaFold DB: A0A172ZAC2

Antitoxin

• AlphaFold DB: A0A172ZA00