Detailed information of TA system TA063237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 9087..9720
• Replicon: plasmid pP27494_2
• Accession: NZ_CP015602
• Organism: Pseudomonas antarctica strain PAMC 27494

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A172ZAE6
• Locus tag: A7J50_RS30090
• Protein ID: WP_064455169.1
• Coordinates: 9319..9720 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: A7J50_RS31255
• Protein ID: WP_082896019.1
• Coordinates: 9087..9326 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7J50_RS30060	4956..5234	-	279	WP_064455139.1	hypothetical protein	-
A7J50_RS30065	5279..5737	-	459	WP_064455140.1	hypothetical protein	-
A7J50_RS30070	5989..6210	-	222	WP_064455141.1	helix-turn-helix domain-containing protein	-
A7J50_RS30080	7147..7959	+	813	WP_156526341.1	plasmid replication protein repA	-
A7J50_RS30085	8723..9019	+	297	WP_064455144.1	hypothetical protein	-
A7J50_RS31255	9087..9326	+	240	WP_082896019.1	antitoxin	Antitoxin
A7J50_RS30090	9319..9720	+	402	WP_064455169.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A7J50_RS30095	9736..10182	+	447	WP_064455145.1	hypothetical protein	-
A7J50_RS30100	10981..11523	+	543	WP_064455146.1	hypothetical protein	-
A7J50_RS30105	11526..12671	+	1146	WP_064455147.1	hypothetical protein	-
A7J50_RS30110	12681..13016	-	336	WP_064455148.1	FxLYD domain-containing protein	-
A7J50_RS30115	13275..13505	+	231	WP_064455149.1	antitoxin	-
A7J50_RS30120	13505..13903	+	399	WP_064455150.1	tRNA(fMet)-specific endonuclease VapC	-
A7J50_RS31260	14155..14334	+	180	WP_082896020.1	CrpP family protein	-
A7J50_RS30125	14331..14543	+	213	WP_064455151.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..30116	30116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14901.22 Da
• Isoelectric Point: 7.3571

>T63237 WP_064455169.1 NZ_CP015602:9319-9720 [Pseudomonas antarctica]
MIKYMLDTNIVIYVIKRRPVEVLEKFNANAGRMVISSITLAELMHGAEKSAFIEKNTRAVEDFISRLDVLNYDEKAACHY
GSIRADLEKKGITIGVNDLHIAGHARSSGLVVVTNNVNEFNRVNGLIVENWIV

Nucleotide

• Length: 402 bp

>T63237 NZ_CP015602:9319-9720 [Pseudomonas antarctica]
ATGATTAAATACATGCTTGATACGAATATTGTAATTTATGTGATCAAGCGTCGGCCTGTCGAGGTGTTGGAAAAATTCAA
TGCCAATGCGGGCCGGATGGTTATTTCGTCAATCACATTAGCCGAACTAATGCACGGTGCAGAAAAAAGCGCTTTCATTG
AAAAGAACACGCGAGCTGTGGAGGACTTCATTTCAAGACTCGACGTTTTGAACTACGATGAAAAGGCAGCCTGCCACTAC
GGCTCGATACGTGCGGACCTGGAGAAAAAAGGTATCACGATAGGGGTTAATGATTTGCATATAGCTGGACATGCCCGAAG
TTCAGGTTTAGTCGTTGTCACGAACAACGTAAATGAATTCAACCGCGTGAACGGTTTAATTGTCGAAAACTGGATTGTTT
GA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9001.95 Da
• Isoelectric Point: 4.3707

>AT63237 WP_082896019.1 NZ_CP015602:9087-9326 [Pseudomonas antarctica]
MSQGTIFTNNRTQNIRIPADMRFPADVKTVIVRAVGHERIIAPIENTWDSFFLGGESVSNDFMNERADQAQSEREPFDD

Nucleotide

• Length: 240 bp

>AT63237 NZ_CP015602:9087-9326 [Pseudomonas antarctica]
ATGTCTCAAGGAACTATATTCACCAATAACCGAACACAAAACATCCGTATCCCTGCGGATATGCGGTTTCCTGCGGATGT
TAAAACAGTCATTGTGCGGGCTGTCGGGCACGAACGCATCATTGCCCCCATTGAAAACACTTGGGATAGTTTTTTCCTTG
GCGGTGAGTCAGTTTCAAACGACTTCATGAATGAACGAGCTGACCAGGCACAAAGCGAAAGAGAACCGTTCGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A172ZAE6