Detailed information of TA system TA063233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1702190..1702805
• Replicon: chromosome
• Accession: NZ_CP015600
• Organism: Pseudomonas antarctica strain PAMC 27494

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: A7J50_RS07790
• Protein ID: WP_064451280.1
• Coordinates: 1702623..1702805 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A172YXH6
• Locus tag: A7J50_RS07785
• Protein ID: WP_064451279.1
• Coordinates: 1702190..1702600 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7J50_RS07745	1697328..1697816	-	489	WP_064451271.1	DMT family transporter	-
A7J50_RS07750	1697885..1698811	+	927	WP_064451272.1	LysR family transcriptional regulator	-
A7J50_RS07755	1698825..1699235	+	411	WP_064451273.1	fosfomycin resistance glutathione transferase	-
A7J50_RS07760	1699290..1699931	+	642	WP_064451274.1	LysE family translocator	-
A7J50_RS07765	1700051..1700578	+	528	WP_064451275.1	DUF4142 domain-containing protein	-
A7J50_RS07770	1700633..1701058	-	426	WP_064451276.1	low affinity iron permease family protein	-
A7J50_RS07775	1701170..1701670	-	501	WP_064451277.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
A7J50_RS07785	1702190..1702600	-	411	WP_064451279.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A7J50_RS07790	1702623..1702805	-	183	WP_064451280.1	type II toxin-antitoxin system HicA family toxin	Toxin
A7J50_RS07795	1702973..1703866	-	894	WP_064451281.1	LysR family transcriptional regulator	-
A7J50_RS07800	1703962..1705125	+	1164	WP_064451282.1	MFS transporter	-
A7J50_RS07805	1705122..1706315	+	1194	WP_064451283.1	MFS transporter	-
A7J50_RS07810	1706505..1706819	+	315	WP_064454872.1	hypothetical protein	-
A7J50_RS07815	1706819..1707112	+	294	WP_064451284.1	helix-turn-helix domain-containing protein	-
A7J50_RS07820	1707132..1707335	-	204	WP_064451285.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6689.86 Da
• Isoelectric Point: 10.8513

>T63233 WP_064451280.1 NZ_CP015600:c1702805-1702623 [Pseudomonas antarctica]
VDSRYLIGHIVADGGYLVRTRGSHHHFKHPKKPGLVTIPHPKKDLLDKTAKSILKQALLS

Nucleotide

• Length: 183 bp

>T63233 NZ_CP015600:c1702805-1702623 [Pseudomonas antarctica]
GTGGATAGTCGATATTTGATAGGTCACATTGTTGCGGACGGCGGGTACCTGGTGCGCACCCGAGGCAGTCATCATCACTT
CAAACACCCGAAAAAACCGGGGTTGGTGACAATTCCTCATCCAAAGAAGGACCTGTTGGACAAAACCGCCAAGAGCATTT
TGAAACAGGCTCTGTTGAGCTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14712.59 Da
• Isoelectric Point: 4.2239

>AT63233 WP_064451279.1 NZ_CP015600:c1702600-1702190 [Pseudomonas antarctica]
MLYPIAISTGDEDHAWGVEVPDIPGCYSAGEDLDDAMAMAREAIEGHFEILAEDGAPIPTAQKVTLHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLNHPEEKSRSGFLASAALKVLQQDG

Nucleotide

• Length: 411 bp

>AT63233 NZ_CP015600:c1702600-1702190 [Pseudomonas antarctica]
ATGCTTTACCCCATCGCAATTTCAACCGGCGACGAGGATCACGCCTGGGGTGTGGAAGTCCCGGATATTCCTGGTTGCTA
CTCAGCCGGGGAAGACCTCGACGACGCCATGGCCATGGCCCGGGAAGCGATCGAAGGGCACTTCGAAATCCTCGCGGAGG
ATGGCGCGCCGATCCCCACCGCGCAAAAAGTTACCCTGCACGCGGCAAACCCACAGTACGCCGGCTGCACGTGGGCCGTG
GTGGACATTGATGTCACCAAGTACCTGGGCAAGGCTCAGAAACTCAACATTACCCTGCCGGGTTATCTGTTGAATCGTAT
TGATGAATACGTGCTGAATCACCCGGAAGAAAAAAGCCGTTCGGGGTTTCTGGCATCCGCGGCGCTCAAGGTATTGCAAC
AGGACGGGTAG

Structures

Antitoxin

• AlphaFold DB: A0A172YXH6