Detailed information of TA system TA063229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 73366..73922
• Replicon: plasmid pMYC1
• Accession: NZ_CP015597
• Organism: Mycobacterium sp. YC-RL4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A172UWA2
• Locus tag: A7U43_RS28075
• Protein ID: WP_068004062.1
• Coordinates: 73366..73689 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A172UWB9
• Locus tag: A7U43_RS28080
• Protein ID: WP_068004064.1
• Coordinates: 73686..73922 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7U43_RS28030	68408..68851	+	444	WP_068004039.1	hypothetical protein	-
A7U43_RS28035	68851..69135	+	285	WP_068004041.1	hypothetical protein	-
A7U43_RS28040	69177..69416	-	240	WP_082902521.1	hypothetical protein	-
A7U43_RS28045	69491..70330	-	840	WP_068004431.1	hypothetical protein	-
A7U43_RS28050	70513..70998	+	486	WP_068004045.1	hypothetical protein	-
A7U43_RS28055	71076..71852	+	777	WP_156526208.1	hypothetical protein	-
A7U43_RS28060	71883..72212	+	330	WP_068004059.1	hypothetical protein	-
A7U43_RS28065	72199..72597	+	399	WP_068004061.1	hypothetical protein	-
A7U43_RS28070	72725..73369	-	645	WP_068004436.1	hypothetical protein	-
A7U43_RS28075	73366..73689	-	324	WP_068004062.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A7U43_RS28080	73686..73922	-	237	WP_068004064.1	antitoxin MazE5	Antitoxin
A7U43_RS28085	73970..74170	-	201	WP_068004067.1	hypothetical protein	-
A7U43_RS28090	74362..75468	+	1107	WP_068004070.1	hypothetical protein	-
A7U43_RS28095	75478..75819	-	342	WP_082902502.1	hypothetical protein	-
A7U43_RS29480	75831..76064	+	234	WP_082902503.1	hypothetical protein	-
A7U43_RS28100	76243..77241	-	999	WP_156526209.1	IS481 family transposase	-
A7U43_RS28105	77307..78395	-	1089	WP_082902504.1	DUF222 domain-containing protein	-
A7U43_RS28110	78548..78739	-	192	WP_024450559.1	(4Fe-4S)-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..252568	252568
	-	flank	IS/Tn	-	-	76243..77310	1067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-102)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11432.34 Da
• Isoelectric Point: 7.6843

>T63229 WP_068004062.1 NZ_CP015597:c73689-73366 [Mycobacterium sp. YC-RL4]
VTLPARGELWWCETTDAAPRPVVVLSRDVAIPRLRRVLVAPCTTTVRGLASEVVLEPGADPVPQRAAVNLDLVESVPVAA
LGSRLGRLADDRMRAVCAALAVAVDWR

Nucleotide

• Length: 324 bp

>T63229 NZ_CP015597:c73689-73366 [Mycobacterium sp. YC-RL4]
GTGACGCTGCCGGCGCGCGGGGAGCTGTGGTGGTGCGAGACCACCGACGCCGCGCCGCGCCCGGTGGTCGTGCTCTCGCG
CGATGTCGCGATTCCCCGGCTGCGCCGTGTCCTGGTCGCGCCGTGCACCACGACGGTGCGCGGGTTGGCCAGTGAGGTCG
TCCTGGAACCGGGCGCGGATCCGGTGCCGCAGCGGGCGGCGGTGAACCTCGATCTGGTCGAGAGCGTGCCGGTGGCGGCG
CTGGGGTCCCGGTTGGGTCGGCTGGCTGACGATCGGATGCGGGCGGTGTGCGCCGCTTTGGCCGTGGCGGTGGACTGGCG
TTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8528.41 Da
• Isoelectric Point: 5.0057

>AT63229 WP_068004064.1 NZ_CP015597:c73922-73686 [Mycobacterium sp. YC-RL4]
MPRTRVSTTVDTDLLDQVRKQMPGAKDHALLDTALAALLAANRAAEVDASYAAYDEHPADEPDHWGDVAAWRQSAGRR

Nucleotide

• Length: 237 bp

>AT63229 NZ_CP015597:c73922-73686 [Mycobacterium sp. YC-RL4]
ATGCCGCGCACACGGGTGAGCACGACGGTGGATACCGATCTGCTGGACCAGGTGCGCAAGCAGATGCCCGGCGCGAAGGA
TCACGCACTGCTCGACACTGCGTTGGCCGCGCTGTTGGCGGCGAACCGGGCCGCCGAGGTCGACGCCAGCTACGCGGCCT
ACGACGAGCATCCGGCCGATGAGCCCGACCACTGGGGCGACGTCGCGGCCTGGCGACAGTCGGCGGGGCGACGGTGA

Structures

Toxin

• AlphaFold DB: A0A172UWA2

Antitoxin

• AlphaFold DB: A0A172UWB9