Detailed information of TA system TA063228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 164597..165181
• Replicon: plasmid unnamed
• Accession: NZ_CP015595
• Organism: Acinetobacter sp. NCu2D-2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: A3K93_RS13940
• Protein ID: WP_000286964.1
• Coordinates: 164879..165181 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: A3K93_RS13935
• Protein ID: WP_000985610.1
• Coordinates: 164597..164878 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3K93_RS13895	160674..160889	+	216	WP_005237341.1	cold-shock protein	-
A3K93_RS13900	160988..161215	+	228	WP_067731910.1	hypothetical protein	-
A3K93_RS13905	161284..161481	+	198	WP_005237334.1	hypothetical protein	-
A3K93_RS13910	161744..162289	-	546	WP_005176794.1	XRE family transcriptional regulator	-
A3K93_RS13915	162382..163260	+	879	WP_067732190.1	DMT family transporter	-
A3K93_RS13920	163339..163632	-	294	WP_067731912.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A3K93_RS13925	163622..163867	-	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
A3K93_RS13930	163961..164488	-	528	WP_000537572.1	plasmid pRiA4b ORF-3 family protein	-
A3K93_RS13935	164597..164878	-	282	WP_000985610.1	putative addiction module antidote protein	Antitoxin
A3K93_RS13940	164879..165181	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A3K93_RS13945	165429..165632	+	204	WP_067731914.1	hypothetical protein	-
A3K93_RS13950	165897..167867	+	1971	WP_067731915.1	choline BCCT transporter BetT	-
A3K93_RS14780	168022..168150	-	129	Protein_152	IS3 family transposase	-
A3K93_RS13955	168531..169463	+	933	WP_081408502.1	IS5-like element IS17 family transposase	-
A3K93_RS13960	169493..169840	-	348	WP_101494841.1	NAD(P)/FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..309964	309964
	-	flank	IS/Tn	-	-	168594..169463	869

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T63228 WP_000286964.1 NZ_CP015595:c165181-164879 [Acinetobacter sp. NCu2D-2]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T63228 NZ_CP015595:c165181-164879 [Acinetobacter sp. NCu2D-2]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTTACTAAGTTAAAAGACCAGCAGGCAAAAAGACGTATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAACCAGTAGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGACCTGGCTACAGGATCTATTACTGTAAGCAAGGGCAAAGGGTCGTTATTCTTTTAGCAGGCGGAGACAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT63228 WP_000985610.1 NZ_CP015595:c164878-164597 [Acinetobacter sp. NCu2D-2]
MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT63228 NZ_CP015595:c164878-164597 [Acinetobacter sp. NCu2D-2]
ATGGCTATCAAACTTCGTAAATGGGACAGTGCTGAACATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCGGCATTCATTGCAAAAGCTTTAGGTAATATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCAAGAGACACTGGTTTAGGTCGAGAAAGTCTTTATAAAGCACTTTCTGGCGATGTAAATCCAAGCTTTGATACTGTT
ATTAAAGTCGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94