Detailed information of TA system TA063227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 163339..163867
• Replicon: plasmid unnamed
• Accession: NZ_CP015595
• Organism: Acinetobacter sp. NCu2D-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A172YSU0
• Locus tag: A3K93_RS13920
• Protein ID: WP_067731912.1
• Coordinates: 163339..163632 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9B5Q7
• Locus tag: A3K93_RS13925
• Protein ID: WP_000246756.1
• Coordinates: 163622..163867 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3K93_RS13880	158829..159048	-	220	Protein_137	hypothetical protein	-
A3K93_RS13885	159168..159488	+	321	WP_067731908.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A3K93_RS13890	159481..159753	+	273	WP_004641921.1	helix-turn-helix domain-containing protein	-
A3K93_RS13895	160674..160889	+	216	WP_005237341.1	cold-shock protein	-
A3K93_RS13900	160988..161215	+	228	WP_067731910.1	hypothetical protein	-
A3K93_RS13905	161284..161481	+	198	WP_005237334.1	hypothetical protein	-
A3K93_RS13910	161744..162289	-	546	WP_005176794.1	XRE family transcriptional regulator	-
A3K93_RS13915	162382..163260	+	879	WP_067732190.1	DMT family transporter	-
A3K93_RS13920	163339..163632	-	294	WP_067731912.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A3K93_RS13925	163622..163867	-	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
A3K93_RS13930	163961..164488	-	528	WP_000537572.1	plasmid pRiA4b ORF-3 family protein	-
A3K93_RS13935	164597..164878	-	282	WP_000985610.1	putative addiction module antidote protein	-
A3K93_RS13940	164879..165181	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A3K93_RS13945	165429..165632	+	204	WP_067731914.1	hypothetical protein	-
A3K93_RS13950	165897..167867	+	1971	WP_067731915.1	choline BCCT transporter BetT	-
A3K93_RS14780	168022..168150	-	129	Protein_152	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..309964	309964
	-	flank	IS/Tn	-	-	168594..169463	869

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-71)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11364.59 Da
• Isoelectric Point: 10.3735

>T63227 WP_067731912.1 NZ_CP015595:c163632-163339 [Acinetobacter sp. NCu2D-2]
MTYKLLRHKDFTAEWEKLPVAIRDQFKKKLAKVIEQPHIPKNMLRGDLAGCYKIKLLKAGVRLVYQVKDDQVVILLITVE
KRADSIVYDEAKKRIKD

Nucleotide

• Length: 294 bp

>T63227 NZ_CP015595:c163632-163339 [Acinetobacter sp. NCu2D-2]
ATGACTTATAAGCTTTTACGTCACAAGGACTTTACAGCAGAATGGGAAAAACTCCCTGTTGCTATACGAGATCAATTTAA
AAAGAAACTAGCTAAAGTCATAGAGCAGCCACATATTCCAAAGAACATGCTTAGAGGCGATCTTGCAGGTTGCTATAAAA
TCAAATTATTAAAAGCGGGTGTCCGACTTGTCTATCAAGTTAAAGATGATCAGGTAGTTATCCTGCTCATTACCGTCGAA
AAACGAGCTGATAGCATAGTTTATGACGAAGCGAAAAAGCGAATTAAAGATTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9235.61 Da
• Isoelectric Point: 4.3142

>AT63227 WP_000246756.1 NZ_CP015595:c163867-163622 [Acinetobacter sp. NCu2D-2]
MATVNFRVDEALKEKSYSILKEQGIAPTDFFTSILEYVATTGKLPVKKALLSEEDEELLALVRKRINDPKEMFEEVTLDD
L

Nucleotide

• Length: 246 bp

>AT63227 NZ_CP015595:c163867-163622 [Acinetobacter sp. NCu2D-2]
ATGGCTACTGTTAACTTCAGGGTCGATGAAGCCCTTAAAGAAAAATCTTATTCAATCCTTAAAGAGCAAGGTATTGCACC
TACGGATTTCTTTACTAGTATTCTTGAGTATGTTGCTACGACTGGGAAACTGCCCGTCAAAAAAGCTTTGCTTTCGGAAG
AAGATGAAGAATTATTAGCCCTTGTCCGTAAGCGCATTAACGATCCTAAAGAGATGTTTGAGGAAGTCACATTAGATGAC
TTATAA

Structures

Toxin

• AlphaFold DB: A0A172YSU0

Antitoxin

• AlphaFold DB: N9B5Q7