Detailed information of TA system TA063225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 157677..158214
• Replicon: plasmid unnamed
• Accession: NZ_CP015595
• Organism: Acinetobacter sp. NCu2D-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N8RL18
• Locus tag: A3K93_RS13865
• Protein ID: WP_004676242.1
• Coordinates: 157677..157967 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: N9QXW3
• Locus tag: A3K93_RS13870
• Protein ID: WP_005265047.1
• Coordinates: 157954..158214 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3K93_RS14775	153429..153518	+	90	Protein_127	MerR family DNA-binding transcriptional regulator	-
A3K93_RS13835	153571..154503	-	933	WP_081408502.1	IS5-like element IS17 family transposase	-
A3K93_RS13840	154632..154892	-	261	WP_005265047.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
A3K93_RS13845	154989..155417	-	429	WP_067731899.1	arsenate reductase (glutaredoxin)	-
A3K93_RS13850	155500..155814	+	315	WP_067731901.1	metalloregulator ArsR/SmtB family transcription factor	-
A3K93_RS13855	155819..156859	+	1041	WP_067731906.1	ACR3 family arsenite efflux transporter	-
A3K93_RS13860	156865..157569	+	705	WP_034583466.1	arsenical resistance protein ArsH	-
A3K93_RS13865	157677..157967	-	291	WP_004676242.1	type II toxin-antitoxin system YafQ family toxin	Toxin
A3K93_RS13870	157954..158214	-	261	WP_005265047.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
A3K93_RS13875	158296..158805	-	510	WP_067731907.1	DinB family protein	-
A3K93_RS13880	158829..159048	-	220	Protein_137	hypothetical protein	-
A3K93_RS13885	159168..159488	+	321	WP_067731908.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A3K93_RS13890	159481..159753	+	273	WP_004641921.1	helix-turn-helix domain-containing protein	-
A3K93_RS13895	160674..160889	+	216	WP_005237341.1	cold-shock protein	-
A3K93_RS13900	160988..161215	+	228	WP_067731910.1	hypothetical protein	-
A3K93_RS13905	161284..161481	+	198	WP_005237334.1	hypothetical protein	-
A3K93_RS13910	161744..162289	-	546	WP_005176794.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..309964	309964

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-94)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-69)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11183.93 Da
• Isoelectric Point: 8.8769

>T63225 WP_004676242.1 NZ_CP015595:c157967-157677 [Acinetobacter sp. NCu2D-2]
MRTIKYTSAFKKDYKREKKGQHRNTVDAELQTVLELLQADEALPEKYRDHALVSNWVGHRDCHIKPDLVLIYKKTEEGEL
LILKLVRLGSHSELAI

Nucleotide

• Length: 291 bp

>T63225 NZ_CP015595:c157967-157677 [Acinetobacter sp. NCu2D-2]
ATGCGGACGATTAAGTACACATCAGCATTCAAGAAAGACTATAAACGTGAGAAAAAAGGGCAGCATCGAAATACAGTAGA
TGCTGAACTACAAACTGTTTTAGAGTTATTGCAAGCTGATGAAGCCTTGCCTGAAAAGTATCGTGACCATGCTTTGGTCA
GTAATTGGGTAGGTCATCGTGACTGCCATATCAAACCAGATCTTGTTTTGATCTATAAAAAAACAGAGGAAGGCGAACTA
CTAATTTTGAAATTAGTTAGGTTAGGTAGTCATAGTGAATTGGCAATTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9363.74 Da
• Isoelectric Point: 4.4354

>AT63225 WP_005265047.1 NZ_CP015595:c158214-157954 [Acinetobacter sp. NCu2D-2]
MASNALVTTRINENIKHEATEVLATMGLTVSDAVRLLLTKVAKEKALPFEIWQPSAETIAAMEEDLSKAKSFTSVKDLMA
DLNADD

Nucleotide

• Length: 261 bp

>AT63225 NZ_CP015595:c158214-157954 [Acinetobacter sp. NCu2D-2]
ATGGCTTCAAATGCACTAGTAACTACTCGCATTAACGAAAACATTAAGCATGAAGCAACTGAAGTTCTTGCAACAATGGG
CTTAACTGTCTCAGATGCCGTTCGCTTGTTGTTAACCAAAGTAGCGAAAGAAAAGGCTCTGCCTTTTGAAATTTGGCAAC
CCAGTGCTGAAACCATTGCTGCAATGGAAGAAGATCTAAGCAAAGCAAAGAGCTTCACTTCTGTAAAAGATTTGATGGCT
GATCTAAATGCGGACGATTAA

Structures

Toxin

• AlphaFold DB: N8RL18

Antitoxin

• AlphaFold DB: N9QXW3