Detailed information of TA system TA063223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 4038305..4039280
• Replicon: chromosome
• Accession: NZ_CP015589
• Organism: Bacillus cereus strain AR156

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: A7A70_RS21590
• Protein ID: WP_061403070.1
• Coordinates: 4038305..4039042 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: D8GY54
• Locus tag: A7A70_RS21595
• Protein ID: WP_000594608.1
• Coordinates: 4039155..4039280 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A70_RS21565	4033341..4033748	+	408	WP_071679614.1	VOC family protein	-
A7A70_RS21570	4034034..4034816	+	783	WP_071679615.1	class I SAM-dependent methyltransferase	-
A7A70_RS21575	4034938..4036623	-	1686	WP_071679616.1	alpha-keto acid decarboxylase family protein	-
A7A70_RS21580	4036730..4037212	+	483	WP_000191902.1	MarR family winged helix-turn-helix transcriptional regulator	-
A7A70_RS21585	4037379..4038116	+	738	WP_071679617.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
A7A70_RS21590	4038305..4039042	+	738	WP_061403070.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
A7A70_RS21595	4039155..4039280	+	126	WP_000594608.1	hypothetical protein	Antitoxin
A7A70_RS21600	4039356..4039532	+	177	WP_000852620.1	stage II sporulation protein SB	-
A7A70_RS21605	4039551..4039940	-	390	WP_071679618.1	YxeA family protein	-
A7A70_RS21610	4040156..4041625	+	1470	WP_071679619.1	beta-Ala-His dipeptidase	-
A7A70_RS21620	4042175..4042645	-	471	WP_071679620.1	DUF5065 family protein	-
A7A70_RS21625	4043100..4043759	+	660	WP_071679621.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28405.13 Da
• Isoelectric Point: 8.2672

>T63223 WP_061403070.1 NZ_CP015589:4038305-4039042 [Bacillus cereus]
VISNIRIGLFILVIVFLVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTMIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T63223 NZ_CP015589:4038305-4039042 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGTAATCGTTTTTCTAGTACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCGAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATACGTTCTCAATTTAGTACTATGATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4878.27 Da
• Isoelectric Point: 3.9507

>AT63223 WP_000594608.1 NZ_CP015589:4039155-4039280 [Bacillus cereus]
VIKNNDNDFFKEDNEDTTDFSLIKGDTMQQVEDLEDELKRK

Nucleotide

• Length: 126 bp

>AT63223 NZ_CP015589:4039155-4039280 [Bacillus cereus]
GTGATAAAAAATAATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACGGATTTCTCGTTAATTAAAGGGGATAC
GATGCAGCAAGTAGAAGATTTAGAAGATGAATTAAAAAGAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U6H9M7