Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1924530..1925172 Replicon chromosome
Accession NZ_CP015589
Organism Bacillus cereus strain AR156

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag A7A70_RS10535 Protein ID WP_000635963.1
Coordinates 1924822..1925172 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag A7A70_RS10530 Protein ID WP_000004570.1
Coordinates 1924530..1924817 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A70_RS10505 1919848..1920810 + 963 WP_061401977.1 UV DNA damage repair endonuclease UvsE -
A7A70_RS10510 1920803..1921375 - 573 WP_071679120.1 rhomboid family intramembrane serine protease -
A7A70_RS10515 1921468..1921827 + 360 WP_000635047.1 holo-ACP synthase -
A7A70_RS10520 1921984..1922934 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
A7A70_RS10525 1923053..1924222 + 1170 WP_000390607.1 alanine racemase -
A7A70_RS10530 1924530..1924817 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
A7A70_RS10535 1924822..1925172 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
A7A70_RS10540 1925240..1927408 + 2169 WP_000426229.1 RNA-binding transcriptional accessory protein -
A7A70_RS10545 1927466..1927582 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
A7A70_RS10550 1927778..1928236 + 459 WP_000344257.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T63222 WP_000635963.1 NZ_CP015589:1924822-1925172 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T63222 NZ_CP015589:1924822-1925172 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT63222 WP_000004570.1 NZ_CP015589:1924530-1924817 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT63222 NZ_CP015589:1924530-1924817 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References