Detailed information of TA system TA063221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 13182..13813
• Replicon: plasmid 3
• Accession: NZ_CP015587
• Organism: Roseomonas gilardii strain U14-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RGI145_RS24405
• Protein ID: WP_083671571.1
• Coordinates: 13182..13592 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2D2H0A1
• Locus tag: RGI145_RS24410
• Protein ID: WP_027283136.1
• Coordinates: 13589..13813 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RGI145_RS24385	8392..8793	+	402	WP_075801118.1	DUF2442 domain-containing protein	-
RGI145_RS24390	9222..9404	-	183	WP_075801119.1	hypothetical protein	-
RGI145_RS24395	9564..10781	+	1218	WP_075801120.1	AAA family ATPase	-
RGI145_RS24400	10774..12879	+	2106	WP_075801121.1	S8 family serine peptidase	-
RGI145_RS24405	13182..13592	-	411	WP_083671571.1	type II toxin-antitoxin system VapC family toxin	Toxin
RGI145_RS24410	13589..13813	-	225	WP_027283136.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RGI145_RS24415	13926..15059	-	1134	WP_075801122.1	site-specific integrase	-
RGI145_RS24420	15323..16447	+	1125	WP_075801123.1	hypothetical protein	-
RGI145_RS24425	16634..16966	-	333	WP_075799516.1	NIPSNAP family protein	-
RGI145_RS24430	16988..18226	-	1239	WP_075799517.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..52461	52461

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

• SpoVT-AbrB domain (9-45)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15041.20 Da
• Isoelectric Point: 7.7493

>T63221 WP_083671571.1 NZ_CP015587:c13592-13182 [Roseomonas gilardii]
VTLRYLLDTNLCIRVLRDRPAGLRGRFNAEAAALCTSDVVLYELLYGAERSARPAETRREVERFVARLSVLPFDDDAAAH
TAEIRASLERRGRVIGPYDLMIAGHARSRGLVVVTGNLGEFGRVEGLRCEDWLAGA

Nucleotide

• Length: 411 bp

>T63221 NZ_CP015587:c13592-13182 [Roseomonas gilardii]
GTGACGCTGCGCTACCTGCTCGACACCAACCTGTGCATCCGGGTGCTGCGTGATCGCCCGGCCGGTCTGCGGGGGCGTTT
CAACGCGGAGGCCGCGGCGCTCTGCACCTCGGACGTGGTGCTCTACGAGCTGCTCTACGGGGCGGAGCGCTCGGCCAGGC
CCGCGGAGACGCGGCGCGAGGTGGAGCGCTTCGTGGCCAGGCTGAGCGTGCTGCCCTTCGACGATGACGCGGCGGCGCAC
ACGGCCGAGATCCGGGCGAGCCTGGAGCGGCGGGGCAGGGTGATCGGTCCCTACGACCTGATGATCGCCGGCCACGCGCG
CAGCCGCGGGCTGGTGGTGGTGACGGGCAACCTCGGCGAATTCGGCCGCGTGGAGGGGCTGCGCTGCGAGGATTGGCTGG
CCGGGGCCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8189.41 Da
• Isoelectric Point: 9.1096

>AT63221 WP_027283136.1 NZ_CP015587:c13813-13589 [Roseomonas gilardii]
MPARTTLFLSNRSQAVRLPKGVAFGEGVREVVILRDGPRRVIAPADALWDDFFAGPGLDLGERRQPAMQEREAL

Nucleotide

• Length: 225 bp

>AT63221 NZ_CP015587:c13813-13589 [Roseomonas gilardii]
ATGCCCGCCCGCACGACCCTGTTCCTGTCCAACCGCTCCCAGGCCGTGCGCCTGCCCAAGGGCGTGGCCTTCGGCGAGGG
CGTGCGCGAGGTGGTGATCCTGCGCGACGGTCCCCGCCGCGTCATCGCCCCGGCTGACGCACTGTGGGACGACTTCTTCG
CAGGCCCTGGCCTGGACCTCGGCGAGCGGCGGCAGCCGGCGATGCAGGAACGCGAGGCGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2D2H0A1