Detailed information of TA system TA063206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 988329..988969
• Replicon: chromosome
• Accession: NZ_CP015571
• Organism: Pasteurella multocida strain USDA-ARS-USMARC-60214

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V4NDJ2
• Locus tag: A4204_RS05175
• Protein ID: WP_016533221.1
• Coordinates: 988793..988969 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V4Q272
• Locus tag: A4204_RS05170
• Protein ID: WP_016533220.1
• Coordinates: 988329..988745 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4204_RS05120	983858..984394	+	537	WP_199764788.1	phage N-6-adenine-methyltransferase	-
A4204_RS05125	984384..984842	+	459	WP_014391471.1	recombination protein NinB	-
A4204_RS05130	984916..985128	+	213	WP_016533468.1	hypothetical protein	-
A4204_RS05135	985216..985818	+	603	WP_016533469.1	recombination protein NinG	-
A4204_RS05140	985818..986183	+	366	WP_016533470.1	hypothetical protein	-
A4204_RS05145	986392..986607	-	216	WP_015691066.1	hypothetical protein	-
A4204_RS05150	986985..987281	+	297	WP_014390733.1	hypothetical protein	-
A4204_RS05155	987278..987808	+	531	WP_016533452.1	lysozyme	-
A4204_RS05160	987781..988104	+	324	WP_075269227.1	DUF2570 family protein	-
A4204_RS05170	988329..988745	-	417	WP_016533220.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A4204_RS05175	988793..988969	-	177	WP_016533221.1	type II toxin-antitoxin system HicA family toxin	Toxin
A4204_RS05180	989231..989707	+	477	WP_075269225.1	DUF1441 family protein	-
A4204_RS05185	989710..991818	+	2109	WP_101750083.1	phage terminase large subunit family protein	-
A4204_RS05190	991815..992036	+	222	WP_014391481.1	hypothetical protein	-
A4204_RS05195	992033..993571	+	1539	WP_016533224.1	phage portal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	966173..1007408	41235

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-78)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6530.60 Da
• Isoelectric Point: 10.7333

>T63206 WP_016533221.1 NZ_CP015571:c988969-988793 [Pasteurella multocida]
MKQSEFLRWLKAQGVETKEGSNHIKLYLNGNQSALPRHPSKEIAKGTEIAVKKQLGLK

Nucleotide

• Length: 177 bp

>T63206 NZ_CP015571:c988969-988793 [Pasteurella multocida]
ATGAAACAAAGTGAGTTTTTAAGATGGCTGAAAGCTCAAGGGGTAGAAACTAAAGAAGGCTCAAACCACATTAAGCTCTA
CCTAAACGGCAACCAATCAGCTCTCCCAAGACATCCAAGCAAAGAGATAGCCAAGGGAACTGAAATAGCAGTTAAAAAGC
AATTAGGTCTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15580.82 Da
• Isoelectric Point: 4.3715

>AT63206 WP_016533220.1 NZ_CP015571:c988745-988329 [Pasteurella multocida]
MLYPAKFDKEDNGLYAVSFRDIPEALTCGDNFDDAVAMAKDALVTSMDFYFEDFRKVPLPTEPEQDEVLIELPDSVFAKV
LLLNEMVEQNISNAELARRINVRPQEMQRITNISHSTKIDTISRALSALGKKLQLSVV

Nucleotide

• Length: 417 bp

>AT63206 NZ_CP015571:c988745-988329 [Pasteurella multocida]
ATGTTATATCCAGCAAAATTTGATAAAGAAGATAATGGGCTTTATGCTGTATCATTTCGTGATATACCTGAAGCCTTAAC
TTGTGGTGATAACTTCGACGATGCCGTCGCAATGGCAAAAGATGCCTTGGTTACATCAATGGATTTTTACTTTGAGGACT
TCCGCAAAGTGCCATTACCAACCGAGCCAGAGCAAGATGAGGTATTAATCGAGTTACCAGATAGTGTTTTTGCTAAAGTG
CTTTTATTAAATGAGATGGTTGAACAAAATATCTCTAATGCTGAATTAGCAAGAAGAATTAATGTTCGACCACAGGAAAT
GCAACGCATTACTAATATCTCGCATAGCACAAAAATCGACACAATAAGCCGTGCTTTGTCAGCACTCGGTAAAAAACTAC
AACTTTCAGTCGTCTAA

Structures

Toxin

• AlphaFold DB: V4NDJ2

Antitoxin

• AlphaFold DB: V4Q272