Detailed information of TA system TA063203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 987732..988372
• Replicon: chromosome
• Accession: NZ_CP015569
• Organism: Pasteurella multocida strain USDA-ARS-USMARC-60385

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V4NDJ2
• Locus tag: A4210_RS05165
• Protein ID: WP_016533221.1
• Coordinates: 988196..988372 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V4Q272
• Locus tag: A4210_RS05160
• Protein ID: WP_016533220.1
• Coordinates: 987732..988148 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4210_RS05110	983261..983797	+	537	WP_199764788.1	phage N-6-adenine-methyltransferase	-
A4210_RS05115	983787..984245	+	459	WP_014391471.1	recombination protein NinB	-
A4210_RS05120	984319..984531	+	213	WP_016533468.1	hypothetical protein	-
A4210_RS05125	984619..985221	+	603	WP_016533469.1	recombination protein NinG	-
A4210_RS05130	985221..985586	+	366	WP_016533470.1	hypothetical protein	-
A4210_RS05135	985795..986010	-	216	WP_015691066.1	hypothetical protein	-
A4210_RS05140	986388..986684	+	297	WP_014390733.1	hypothetical protein	-
A4210_RS05145	986681..987211	+	531	WP_016533452.1	lysozyme	-
A4210_RS05150	987184..987507	+	324	WP_075269227.1	DUF2570 family protein	-
A4210_RS05160	987732..988148	-	417	WP_016533220.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A4210_RS05165	988196..988372	-	177	WP_016533221.1	type II toxin-antitoxin system HicA family toxin	Toxin
A4210_RS05170	988634..989110	+	477	WP_075269225.1	DUF1441 family protein	-
A4210_RS05175	989113..991221	+	2109	WP_101750083.1	phage terminase large subunit family protein	-
A4210_RS05180	991218..991439	+	222	WP_014391481.1	hypothetical protein	-
A4210_RS05185	991436..992974	+	1539	WP_016533224.1	phage portal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	965576..1006811	41235

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-78)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6530.60 Da
• Isoelectric Point: 10.7333

>T63203 WP_016533221.1 NZ_CP015569:c988372-988196 [Pasteurella multocida]
MKQSEFLRWLKAQGVETKEGSNHIKLYLNGNQSALPRHPSKEIAKGTEIAVKKQLGLK

Nucleotide

• Length: 177 bp

>T63203 NZ_CP015569:c988372-988196 [Pasteurella multocida]
ATGAAACAAAGTGAGTTTTTAAGATGGCTGAAAGCTCAAGGGGTAGAAACTAAAGAAGGCTCAAACCACATTAAGCTCTA
CCTAAACGGCAACCAATCAGCTCTCCCAAGACATCCAAGCAAAGAGATAGCCAAGGGAACTGAAATAGCAGTTAAAAAGC
AATTAGGTCTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15580.82 Da
• Isoelectric Point: 4.3715

>AT63203 WP_016533220.1 NZ_CP015569:c988148-987732 [Pasteurella multocida]
MLYPAKFDKEDNGLYAVSFRDIPEALTCGDNFDDAVAMAKDALVTSMDFYFEDFRKVPLPTEPEQDEVLIELPDSVFAKV
LLLNEMVEQNISNAELARRINVRPQEMQRITNISHSTKIDTISRALSALGKKLQLSVV

Nucleotide

• Length: 417 bp

>AT63203 NZ_CP015569:c988148-987732 [Pasteurella multocida]
ATGTTATATCCAGCAAAATTTGATAAAGAAGATAATGGGCTTTATGCTGTATCATTTCGTGATATACCTGAAGCCTTAAC
TTGTGGTGATAACTTCGACGATGCCGTCGCAATGGCAAAAGATGCCTTGGTTACATCAATGGATTTTTACTTTGAGGACT
TCCGCAAAGTGCCATTACCAACCGAGCCAGAGCAAGATGAGGTATTAATCGAGTTACCAGATAGTGTTTTTGCTAAAGTG
CTTTTATTAAATGAGATGGTTGAACAAAATATCTCTAATGCTGAATTAGCAAGAAGAATTAATGTTCGACCACAGGAAAT
GCAACGCATTACTAATATCTCGCATAGCACAAAAATCGACACAATAAGCCGTGCTTTGTCAGCACTCGGTAAAAAACTAC
AACTTTCAGTCGTCTAA

Structures

Toxin

• AlphaFold DB: V4NDJ2

Antitoxin

• AlphaFold DB: V4Q272