Detailed information of TA system TA063201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2012551..2014195
• Replicon: chromosome
• Accession: NZ_CP015568
• Organism: Pasteurella multocida strain USDA-ARS-USMARC-60248

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: V4NI19
• Locus tag: A4207_RS09380
• Protein ID: WP_005752891.1
• Coordinates: 2012888..2014195 (-)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V4NAB0
• Locus tag: A4207_RS09375
• Protein ID: WP_005752888.1
• Coordinates: 2012551..2012886 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4207_RS09350	2008629..2009288	+	660	WP_005724855.1	bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA	-
A4207_RS09355	2009352..2009921	+	570	WP_014326417.1	Der GTPase-activating protein YihI	-
A4207_RS09360	2009927..2010355	+	429	WP_005719208.1	DUF2489 domain-containing protein	-
A4207_RS09365	2010379..2011746	+	1368	WP_016504414.1	oxygen-independent coproporphyrinogen III oxidase	-
A4207_RS09370	2011787..2012455	+	669	WP_014326419.1	NAD(P)H-dependent oxidoreductase	-
A4207_RS09375	2012551..2012886	-	336	WP_005752888.1	helix-turn-helix domain-containing protein	Antitoxin
A4207_RS09380	2012888..2014195	-	1308	WP_005752891.1	HipA domain-containing protein	Toxin
A4207_RS09385	2014414..2015550	+	1137	WP_075266242.1	anhydro-N-acetylmuramic acid kinase	-
A4207_RS09390	2015616..2018198	-	2583	WP_014326421.1	DNA mismatch repair protein MutS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-375)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-94)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 50526.31 Da
• Isoelectric Point: 4.9118

>T63201 WP_005752891.1 NZ_CP015568:c2014195-2012888 [Pasteurella multocida]
MEKLTLQIYLEQAWCDLAFLYFDSETYQLCNFEYLTDYVVQHYGKTGEHAVSLNYPVNVFIDDTTSLWSWLSDIAPAGAS
RRYWLEALDIKQESEEKQQFLLLKHAAISPIGNLRIKDAVPEQVEVRLFDLDKAKDRQSDFLEYVNQSGAMVGGATGAGG
EAPKLLLKRRENQVWIDNQQQGQDQDVSYLVKYPRGNYSPIDCDILRAEYHYYQELAQLGFDTIDTTRMRLEEGKRYPSL
WLPRFDIVQREGKTHRLAFESVYSLLQKQGGVLDHETTLRQLIERISTARLQFAQTDFVIEWVKRDLLNIAFGNSDNHGR
NMAFLRDEQRIWLAPIYDFAPMRADPEGIIRTTTWQTKGEYPQEFAGEYHFERIAEVLADLVEPTLLMQELRRLAGSLVD
LKSRLVQRNLPQSILDFPAIGFDYLPEKLKKWGLI

Nucleotide

• Length: 1308 bp

>T63201 NZ_CP015568:c2014195-2012888 [Pasteurella multocida]
ATGGAAAAACTTACACTGCAAATTTATCTTGAACAAGCTTGGTGCGATTTAGCTTTCTTGTATTTTGATTCAGAAACCTA
TCAACTGTGTAATTTCGAATACTTAACAGATTACGTGGTTCAGCACTATGGAAAAACGGGTGAACATGCTGTGTCGCTTA
ATTATCCAGTGAATGTTTTCATTGATGACACGACGTCGCTGTGGAGCTGGCTCAGTGATATCGCGCCTGCGGGAGCAAGT
CGTCGTTATTGGTTAGAAGCGTTAGACATTAAACAAGAATCAGAGGAAAAACAACAGTTTCTTTTACTGAAACATGCTGC
GATTTCCCCAATTGGCAATTTACGTATCAAAGATGCTGTGCCAGAACAAGTTGAAGTTCGCTTATTTGATCTAGACAAGG
CGAAAGATAGACAGAGTGATTTTTTAGAGTATGTGAACCAAAGTGGTGCGATGGTAGGAGGTGCGACAGGGGCTGGCGGC
GAAGCACCAAAATTGCTTCTTAAACGGCGAGAGAATCAAGTTTGGATTGATAACCAACAGCAAGGGCAGGATCAGGATGT
GAGTTATTTAGTGAAGTACCCACGTGGCAATTACTCACCCATTGATTGTGATATTTTACGTGCAGAATATCATTATTATC
AGGAACTTGCTCAACTGGGTTTTGACACAATAGATACGACTAGAATGAGGTTAGAAGAAGGAAAACGTTATCCTTCCCTA
TGGTTGCCTCGTTTTGATATTGTGCAACGAGAAGGAAAAACACATCGTCTTGCTTTTGAATCAGTGTATTCGCTATTACA
GAAACAAGGTGGTGTACTTGATCATGAAACGACACTTCGCCAGTTAATTGAGAGAATCTCGACGGCTCGTTTGCAGTTTG
CACAAACAGACTTCGTGATTGAATGGGTAAAGCGAGATTTGTTAAATATTGCCTTTGGCAATAGCGACAATCATGGTCGT
AATATGGCGTTTTTACGTGATGAACAACGTATTTGGCTCGCCCCAATTTATGATTTTGCGCCGATGCGTGCTGATCCAGA
AGGCATTATTCGTACGACTACTTGGCAAACTAAAGGGGAGTATCCACAGGAATTTGCTGGCGAGTATCACTTTGAGCGCA
TCGCAGAAGTATTAGCTGATTTAGTTGAACCAACGTTATTAATGCAAGAGTTACGCCGGTTAGCTGGCAGTTTAGTGGAT
TTAAAATCCCGATTAGTACAACGTAATCTACCTCAATCTATTTTAGATTTCCCTGCGATTGGTTTTGATTATTTACCTGA
AAAATTAAAAAAATGGGGGCTTATTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12520.60 Da
• Isoelectric Point: 10.5259

>AT63201 WP_005752888.1 NZ_CP015568:c2012886-2012551 [Pasteurella multocida]
MQITRRNASTKGASVAEIDRKKVRNEIIQAMLMGELSQGEALRKLRIEMFGINQQHYIQLVKVSRQTLSNIENDKGNYSV
ETINKVFKPFGLELGLVPVSKALLKELFSTA

Nucleotide

• Length: 336 bp

>AT63201 NZ_CP015568:c2012886-2012551 [Pasteurella multocida]
ATGCAAATCACTCGAAGAAATGCCTCTACCAAAGGGGCAAGTGTTGCGGAAATTGATCGTAAGAAAGTACGTAATGAAAT
CATTCAAGCGATGCTAATGGGGGAATTGAGCCAAGGTGAAGCATTGCGTAAATTGCGTATTGAAATGTTCGGTATTAATC
AACAACACTATATTCAGTTAGTAAAGGTATCACGCCAGACCCTATCGAATATTGAGAATGATAAGGGTAATTATAGTGTA
GAAACGATTAATAAAGTGTTTAAACCCTTTGGTCTGGAACTCGGCTTGGTACCTGTTTCTAAGGCATTATTAAAGGAACT
TTTTTCGACAGCTTAA

Structures

Toxin

• AlphaFold DB: V4NI19

Antitoxin

• AlphaFold DB: V4NAB0