Detailed information of TA system TA063200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2224435..2226079
• Replicon: chromosome
• Accession: NZ_CP015567
• Organism: Pasteurella multocida strain USDA-ARS-USMARC-60675

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: V4NI19
• Locus tag: A4212_RS10870
• Protein ID: WP_005752891.1
• Coordinates: 2224772..2226079 (-)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V4NAB0
• Locus tag: A4212_RS10865
• Protein ID: WP_005752888.1
• Coordinates: 2224435..2224770 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4212_RS10840	2220513..2221172	+	660	WP_005724855.1	bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA	-
A4212_RS10845	2221236..2221805	+	570	WP_014326417.1	Der GTPase-activating protein YihI	-
A4212_RS10850	2221811..2222239	+	429	WP_005719208.1	DUF2489 domain-containing protein	-
A4212_RS10855	2222263..2223630	+	1368	WP_016504414.1	oxygen-independent coproporphyrinogen III oxidase	-
A4212_RS10860	2223671..2224339	+	669	WP_014326419.1	NAD(P)H-dependent oxidoreductase	-
A4212_RS10865	2224435..2224770	-	336	WP_005752888.1	helix-turn-helix domain-containing protein	Antitoxin
A4212_RS10870	2224772..2226079	-	1308	WP_005752891.1	HipA domain-containing protein	Toxin
A4212_RS10875	2226298..2227434	+	1137	WP_075266242.1	anhydro-N-acetylmuramic acid kinase	-
A4212_RS10880	2227500..2230082	-	2583	WP_014326421.1	DNA mismatch repair protein MutS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-375)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-94)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 50526.31 Da
• Isoelectric Point: 4.9118

>T63200 WP_005752891.1 NZ_CP015567:c2226079-2224772 [Pasteurella multocida]
MEKLTLQIYLEQAWCDLAFLYFDSETYQLCNFEYLTDYVVQHYGKTGEHAVSLNYPVNVFIDDTTSLWSWLSDIAPAGAS
RRYWLEALDIKQESEEKQQFLLLKHAAISPIGNLRIKDAVPEQVEVRLFDLDKAKDRQSDFLEYVNQSGAMVGGATGAGG
EAPKLLLKRRENQVWIDNQQQGQDQDVSYLVKYPRGNYSPIDCDILRAEYHYYQELAQLGFDTIDTTRMRLEEGKRYPSL
WLPRFDIVQREGKTHRLAFESVYSLLQKQGGVLDHETTLRQLIERISTARLQFAQTDFVIEWVKRDLLNIAFGNSDNHGR
NMAFLRDEQRIWLAPIYDFAPMRADPEGIIRTTTWQTKGEYPQEFAGEYHFERIAEVLADLVEPTLLMQELRRLAGSLVD
LKSRLVQRNLPQSILDFPAIGFDYLPEKLKKWGLI

Nucleotide

• Length: 1308 bp

>T63200 NZ_CP015567:c2226079-2224772 [Pasteurella multocida]
ATGGAAAAACTTACACTGCAAATTTATCTTGAACAAGCTTGGTGCGATTTAGCTTTCTTGTATTTTGATTCAGAAACCTA
TCAACTGTGTAATTTCGAATACTTAACAGATTACGTGGTTCAGCACTATGGAAAAACGGGTGAACATGCTGTGTCGCTTA
ATTATCCAGTGAATGTTTTCATTGATGACACGACGTCGCTGTGGAGCTGGCTCAGTGATATCGCGCCTGCGGGAGCAAGT
CGTCGTTATTGGTTAGAAGCGTTAGACATTAAACAAGAATCAGAGGAAAAACAACAGTTTCTTTTACTGAAACATGCTGC
GATTTCCCCAATTGGCAATTTACGTATCAAAGATGCTGTGCCAGAACAAGTTGAAGTTCGCTTATTTGATCTAGACAAGG
CGAAAGATAGACAGAGTGATTTTTTAGAGTATGTGAACCAAAGTGGTGCGATGGTAGGAGGTGCGACAGGGGCTGGCGGC
GAAGCACCAAAATTGCTTCTTAAACGGCGAGAGAATCAAGTTTGGATTGATAACCAACAGCAAGGGCAGGATCAGGATGT
GAGTTATTTAGTGAAGTACCCACGTGGCAATTACTCACCCATTGATTGTGATATTTTACGTGCAGAATATCATTATTATC
AGGAACTTGCTCAACTGGGTTTTGACACAATAGATACGACTAGAATGAGGTTAGAAGAAGGAAAACGTTATCCTTCCCTA
TGGTTGCCTCGTTTTGATATTGTGCAACGAGAAGGAAAAACACATCGTCTTGCTTTTGAATCAGTGTATTCGCTATTACA
GAAACAAGGTGGTGTACTTGATCATGAAACGACACTTCGCCAGTTAATTGAGAGAATCTCGACGGCTCGTTTGCAGTTTG
CACAAACAGACTTCGTGATTGAATGGGTAAAGCGAGATTTGTTAAATATTGCCTTTGGCAATAGCGACAATCATGGTCGT
AATATGGCGTTTTTACGTGATGAACAACGTATTTGGCTCGCCCCAATTTATGATTTTGCGCCGATGCGTGCTGATCCAGA
AGGCATTATTCGTACGACTACTTGGCAAACTAAAGGGGAGTATCCACAGGAATTTGCTGGCGAGTATCACTTTGAGCGCA
TCGCAGAAGTATTAGCTGATTTAGTTGAACCAACGTTATTAATGCAAGAGTTACGCCGGTTAGCTGGCAGTTTAGTGGAT
TTAAAATCCCGATTAGTACAACGTAATCTACCTCAATCTATTTTAGATTTCCCTGCGATTGGTTTTGATTATTTACCTGA
AAAATTAAAAAAATGGGGGCTTATTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12520.60 Da
• Isoelectric Point: 10.5259

>AT63200 WP_005752888.1 NZ_CP015567:c2224770-2224435 [Pasteurella multocida]
MQITRRNASTKGASVAEIDRKKVRNEIIQAMLMGELSQGEALRKLRIEMFGINQQHYIQLVKVSRQTLSNIENDKGNYSV
ETINKVFKPFGLELGLVPVSKALLKELFSTA

Nucleotide

• Length: 336 bp

>AT63200 NZ_CP015567:c2224770-2224435 [Pasteurella multocida]
ATGCAAATCACTCGAAGAAATGCCTCTACCAAAGGGGCAAGTGTTGCGGAAATTGATCGTAAGAAAGTACGTAATGAAAT
CATTCAAGCGATGCTAATGGGGGAATTGAGCCAAGGTGAAGCATTGCGTAAATTGCGTATTGAAATGTTCGGTATTAATC
AACAACACTATATTCAGTTAGTAAAGGTATCACGCCAGACCCTATCGAATATTGAGAATGATAAGGGTAATTATAGTGTA
GAAACGATTAATAAAGTGTTTAAACCCTTTGGTCTGGAACTCGGCTTGGTACCTGTTTCTAAGGCATTATTAAAGGAACT
TTTTTCGACAGCTTAA

Structures

Toxin

• AlphaFold DB: V4NI19

Antitoxin

• AlphaFold DB: V4NAB0