Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 969000..969514 Replicon chromosome
Accession NZ_CP015537
Organism Staphylococcus carnosus strain TMW 2.269

Toxin (Protein)


Gene name mazF Uniprot ID A0A3G4R0E2
Locus tag A2I64_RS04555 Protein ID WP_075140914.1
Coordinates 969164..969514 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID B9DMI8
Locus tag A2I64_RS04550 Protein ID WP_015900820.1
Coordinates 969000..969167 (+) Length 56 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A2I64_RS04525 (A2I64_04430) 964906..965376 + 471 WP_015900825.1 PH domain-containing protein -
A2I64_RS04530 (A2I64_04435) 965369..966856 + 1488 WP_015900824.1 PH domain-containing protein -
A2I64_RS04535 (A2I64_04440) 966846..967346 + 501 WP_115314364.1 PH domain-containing protein -
A2I64_RS04540 (A2I64_04445) 967348..967701 + 354 WP_015900822.1 holo-ACP synthase -
A2I64_RS04545 (A2I64_04450) 967765..968919 + 1155 WP_015900821.1 alanine racemase -
A2I64_RS04550 (A2I64_04455) 969000..969167 + 168 WP_015900820.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
A2I64_RS04555 (A2I64_04460) 969164..969514 + 351 WP_075140914.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
A2I64_RS04560 (A2I64_04465) 969858..970859 + 1002 WP_015900818.1 PP2C family protein-serine/threonine phosphatase -
A2I64_RS04565 (A2I64_04470) 970935..971261 + 327 WP_015900817.1 anti-sigma factor antagonist -
A2I64_RS04570 (A2I64_04475) 971263..971742 + 480 WP_015900816.1 anti-sigma B factor RsbW -
A2I64_RS04575 (A2I64_04480) 971717..972487 + 771 WP_015900815.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13075.18 Da        Isoelectric Point: 10.0708

>T63177 WP_075140914.1 NZ_CP015537:969164-969514 [Staphylococcus carnosus]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIQISLGLTHRN

Download         Length: 351 bp

>T63177 NZ_CP015537:969164-969514 [Staphylococcus carnosus]
ATGATGAGGAGAGGCGATGTTTACTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGGCCTGTTGT
AATTATACAAAATGACACTGGAAATAAGTATAGTCCGACAGTTATTGTGGCAGCAATTACTGGACGGATTAATAAAGCAA
AAATTCCTACTCATGTTGAAATTGAAAAAAGTAAATATAAATTGGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTCGATAAAAAGCGACTTAAAGAAAAACTGACATATTTATCAGACGAAAAGATGAAAGAAATTGATAATGCGATTCA
AATCAGCTTAGGCTTGACACATCGCAATTAA

Antitoxin


Download         Length: 56 a.a.        Molecular weight: 6211.75 Da        Isoelectric Point: 3.6836

>AT63177 WP_015900820.1 NZ_CP015537:969000-969167 [Staphylococcus carnosus]
MSTFNQNRSYSLEQSLKEGYAQMADLNLSLATEAFSVECEACDCNESYLAFDKDE

Download         Length: 168 bp

>AT63177 NZ_CP015537:969000-969167 [Staphylococcus carnosus]
ATGTCAACTTTTAATCAGAATAGAAGTTATAGTTTAGAACAGTCACTGAAAGAAGGCTATGCACAGATGGCTGACTTAAA
TCTCTCCCTCGCAACGGAGGCATTTTCAGTAGAATGTGAAGCCTGTGATTGCAATGAATCATATTTAGCTTTTGACAAGG
ATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G4R0E2

Antitoxin

Source ID Structure
AlphaFold DB A0A380F5Y4

References