Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 968998..969512 Replicon chromosome
Accession NZ_CP015536
Organism Staphylococcus carnosus strain TMW 2.243

Toxin (Protein)


Gene name mazF Uniprot ID A0A3G4R0E2
Locus tag A2I63_RS04550 Protein ID WP_075140914.1
Coordinates 969162..969512 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID B9DMI8
Locus tag A2I63_RS04545 Protein ID WP_015900820.1
Coordinates 968998..969165 (+) Length 56 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A2I63_RS04520 (A2I63_04425) 964904..965374 + 471 WP_015900825.1 PH domain-containing protein -
A2I63_RS04525 (A2I63_04430) 965367..966854 + 1488 WP_015900824.1 PH domain-containing protein -
A2I63_RS04530 (A2I63_04435) 966844..967344 + 501 WP_115314364.1 PH domain-containing protein -
A2I63_RS04535 (A2I63_04440) 967346..967699 + 354 WP_015900822.1 holo-ACP synthase -
A2I63_RS04540 (A2I63_04445) 967763..968917 + 1155 WP_015900821.1 alanine racemase -
A2I63_RS04545 (A2I63_04450) 968998..969165 + 168 WP_015900820.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
A2I63_RS04550 (A2I63_04455) 969162..969512 + 351 WP_075140914.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
A2I63_RS04555 (A2I63_04460) 969856..970857 + 1002 WP_015900818.1 PP2C family protein-serine/threonine phosphatase -
A2I63_RS04560 (A2I63_04465) 970933..971259 + 327 WP_015900817.1 anti-sigma factor antagonist -
A2I63_RS04565 (A2I63_04470) 971261..971740 + 480 WP_015900816.1 anti-sigma B factor RsbW -
A2I63_RS04570 (A2I63_04475) 971715..972485 + 771 WP_015900815.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13075.18 Da        Isoelectric Point: 10.0708

>T63174 WP_075140914.1 NZ_CP015536:969162-969512 [Staphylococcus carnosus]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIQISLGLTHRN

Download         Length: 351 bp

>T63174 NZ_CP015536:969162-969512 [Staphylococcus carnosus]
ATGATGAGGAGAGGCGATGTTTACTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGGCCTGTTGT
AATTATACAAAATGACACTGGAAATAAGTATAGTCCGACAGTTATTGTGGCAGCAATTACTGGACGGATTAATAAAGCAA
AAATTCCTACTCATGTTGAAATTGAAAAAAGTAAATATAAATTGGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTCGATAAAAAGCGACTTAAAGAAAAACTGACATATTTATCAGACGAAAAGATGAAAGAAATTGATAATGCGATTCA
AATCAGCTTAGGCTTGACACATCGCAATTAA

Antitoxin


Download         Length: 56 a.a.        Molecular weight: 6211.75 Da        Isoelectric Point: 3.6836

>AT63174 WP_015900820.1 NZ_CP015536:968998-969165 [Staphylococcus carnosus]
MSTFNQNRSYSLEQSLKEGYAQMADLNLSLATEAFSVECEACDCNESYLAFDKDE

Download         Length: 168 bp

>AT63174 NZ_CP015536:968998-969165 [Staphylococcus carnosus]
ATGTCAACTTTTAATCAGAATAGAAGTTATAGTTTAGAACAGTCACTGAAAGAAGGCTATGCACAGATGGCTGACTTAAA
TCTCTCCCTCGCAACGGAGGCATTTTCAGTAGAATGTGAAGCCTGTGATTGCAATGAATCATATTTAGCTTTTGACAAGG
ATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G4R0E2

Antitoxin

Source ID Structure
AlphaFold DB A0A380F5Y4

References