Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1018842..1019356 Replicon chromosome
Accession NZ_CP015535
Organism Staphylococcus carnosus strain TMW 2.218

Toxin (Protein)


Gene name mazF Uniprot ID A0A3G4R0E2
Locus tag A2I66_RS04870 Protein ID WP_075140914.1
Coordinates 1019006..1019356 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID B9DMI8
Locus tag A2I66_RS04865 Protein ID WP_015900820.1
Coordinates 1018842..1019009 (+) Length 56 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A2I66_RS04840 1014991..1015218 + 228 WP_253720618.1 PH domain-containing protein -
A2I66_RS04845 (A2I66_04755) 1015211..1016698 + 1488 WP_015900824.1 PH domain-containing protein -
A2I66_RS04850 (A2I66_04760) 1016688..1017188 + 501 WP_231635412.1 PH domain-containing protein -
A2I66_RS04855 (A2I66_04765) 1017190..1017543 + 354 WP_015900822.1 holo-ACP synthase -
A2I66_RS04860 (A2I66_04770) 1017607..1018761 + 1155 WP_065865267.1 alanine racemase -
A2I66_RS04865 (A2I66_04775) 1018842..1019009 + 168 WP_015900820.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
A2I66_RS04870 (A2I66_04780) 1019006..1019356 + 351 WP_075140914.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
A2I66_RS04875 (A2I66_04785) 1019700..1020701 + 1002 WP_015900818.1 PP2C family protein-serine/threonine phosphatase -
A2I66_RS04880 (A2I66_04790) 1020777..1021103 + 327 WP_015900817.1 anti-sigma factor antagonist -
A2I66_RS04885 (A2I66_04795) 1021105..1021584 + 480 WP_015900816.1 anti-sigma B factor RsbW -
A2I66_RS04890 (A2I66_04800) 1021559..1022329 + 771 WP_015900815.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13075.18 Da        Isoelectric Point: 10.0708

>T63171 WP_075140914.1 NZ_CP015535:1019006-1019356 [Staphylococcus carnosus]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIQISLGLTHRN

Download         Length: 351 bp

>T63171 NZ_CP015535:1019006-1019356 [Staphylococcus carnosus]
ATGATGAGGAGAGGCGATGTTTACTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGGCCTGTTGT
AATTATACAAAATGACACTGGAAATAAGTATAGTCCGACAGTTATTGTGGCAGCAATTACTGGACGGATTAATAAAGCAA
AAATTCCTACTCATGTTGAAATTGAAAAAAGTAAATATAAATTGGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTCGATAAAAAGCGACTTAAAGAAAAACTGACATATTTATCAGACGAAAAGATGAAAGAAATTGATAATGCGATTCA
AATCAGCTTAGGCTTGACACATCGCAATTAA

Antitoxin


Download         Length: 56 a.a.        Molecular weight: 6211.75 Da        Isoelectric Point: 3.6836

>AT63171 WP_015900820.1 NZ_CP015535:1018842-1019009 [Staphylococcus carnosus]
MSTFNQNRSYSLEQSLKEGYAQMADLNLSLATEAFSVECEACDCNESYLAFDKDE

Download         Length: 168 bp

>AT63171 NZ_CP015535:1018842-1019009 [Staphylococcus carnosus]
ATGTCAACTTTTAATCAGAATAGAAGTTATAGTTTAGAACAGTCACTGAAAGAAGGCTATGCACAGATGGCTGACTTAAA
TCTCTCCCTAGCAACGGAGGCATTTTCAGTAGAATGTGAAGCCTGTGATTGCAATGAATCATATTTAGCTTTTGACAAGG
ACGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G4R0E2

Antitoxin

Source ID Structure
AlphaFold DB A0A380F5Y4

References