Detailed information of TA system TA063170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 1005483..1005994
• Replicon: chromosome
• Accession: NZ_CP015535
• Organism: Staphylococcus carnosus strain TMW 2.218

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: B9DMH5
• Locus tag: A2I66_RS04800
• Protein ID: WP_015900832.1
• Coordinates: 1005788..1005994 (+)
• Length: 69 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: -
• Locus tag: A2I66_RS04795
• Protein ID: WP_065865261.1
• Coordinates: 1005483..1005776 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2I66_RS04770 (A2I66_04685)	1000855..1001493	+	639	WP_015900838.1	thiamine phosphate synthase	-
A2I66_RS04775 (A2I66_04690)	1001582..1002451	+	870	WP_065865259.1	membrane protein insertase YidC	-
A2I66_RS04780 (A2I66_04695)	1002562..1003074	+	513	WP_015900836.1	GNAT family N-acetyltransferase	-
A2I66_RS04785 (A2I66_04700)	1003165..1003812	-	648	WP_065865260.1	HD domain-containing protein	-
A2I66_RS04790 (A2I66_04705)	1003837..1005318	-	1482	WP_253720563.1	cardiolipin synthase	-
A2I66_RS04795 (A2I66_04710)	1005483..1005776	+	294	WP_065865261.1	copper-sensing transcriptional repressor CsoR	Antitoxin
A2I66_RS04800 (A2I66_04715)	1005788..1005994	+	207	WP_015900832.1	heavy metal-associated domain-containing protein	Toxin
A2I66_RS04805 (A2I66_04720)	1006009..1006218	+	210	WP_015900831.1	copper ion binding protein	-
A2I66_RS04810 (A2I66_04725)	1006315..1008516	+	2202	WP_065865262.1	heavy metal translocating P-type ATPase	-
A2I66_RS04815	1008592..1008732	+	141	WP_167313208.1	Lmo0850 family protein	-
A2I66_RS04820 (A2I66_04730)	1008804..1010021	-	1218	WP_015900829.1	FtsW/RodA/SpoVE family cell cycle protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (27-65)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7833.72 Da
• Isoelectric Point: 4.1353

>T63170 WP_015900832.1 NZ_CP015535:1005788-1005994 [Staphylococcus carnosus]
MQNSVIKIDGMETEEEQHKLHQHLIEMTGVSAVQVDMDSNEVRISYETPVNLNNLEKEIYDAGYQILN

Nucleotide

• Length: 207 bp

>T63170 NZ_CP015535:1005788-1005994 [Staphylococcus carnosus]
ATGCAAAATAGTGTAATTAAAATTGATGGAATGGAAACTGAAGAAGAACAGCATAAATTGCATCAACATCTTATAGAAAT
GACAGGTGTATCTGCAGTGCAAGTGGATATGGATTCAAATGAAGTCAGAATCAGTTATGAAACACCTGTAAATCTCAATA
ATCTTGAAAAAGAAATCTATGATGCAGGTTATCAAATATTAAATTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11132.76 Da
• Isoelectric Point: 6.4862

>AT63170 WP_065865261.1 NZ_CP015535:1005483-1005776 [Staphylococcus carnosus]
MTENDKAHHSEQLKSNLKARLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAVRLLDHHMKGCIMHKVEDGHA
EEAMEELLVTVQKLIKD

Nucleotide

• Length: 294 bp

>AT63170 NZ_CP015535:1005483-1005776 [Staphylococcus carnosus]
ATGACCGAAAATGATAAAGCGCACCATTCAGAACAACTCAAATCCAACTTGAAAGCACGACTCAATCGAATTGAAGGACA
AGTCAGAGCAATTAACCGAATGATTGAAGAGGATGTATATTGTGATGATGTACTCACACAAATCCGTGCAACACGTTCAG
CTTTGAACAGTGTGGCAGTACGATTGCTTGATCATCACATGAAAGGTTGTATCATGCATAAAGTTGAGGATGGTCACGCT
GAAGAAGCGATGGAAGAATTGCTTGTTACCGTTCAAAAATTGATTAAAGATTAA

Structures

Toxin

• AlphaFold DB: A0A380F432