Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 1648988..1649499 Replicon chromosome
Accession NZ_CP015533
Organism Staphylococcus carnosus strain TMW 2.216

Toxin (Protein)


Gene name TsdT Uniprot ID B9DMH5
Locus tag A2I67_RS08460 Protein ID WP_015900832.1
Coordinates 1648988..1649194 (-) Length 69 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID B9DMH6
Locus tag A2I67_RS08465 Protein ID WP_015900833.1
Coordinates 1649206..1649499 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A2I67_RS08440 (A2I67_08270) 1644961..1646178 + 1218 WP_015900829.1 FtsW/RodA/SpoVE family cell cycle protein -
A2I67_RS08445 1646250..1646390 - 141 WP_167313208.1 Lmo0850 family protein -
A2I67_RS08450 (A2I67_08275) 1646466..1648667 - 2202 WP_103211725.1 heavy metal translocating P-type ATPase -
A2I67_RS08455 (A2I67_08280) 1648764..1648973 - 210 WP_015900831.1 copper ion binding protein -
A2I67_RS08460 (A2I67_08285) 1648988..1649194 - 207 WP_015900832.1 heavy metal-associated domain-containing protein Toxin
A2I67_RS08465 (A2I67_08290) 1649206..1649499 - 294 WP_015900833.1 copper-sensing transcriptional repressor CsoR Antitoxin
A2I67_RS08470 (A2I67_08295) 1649664..1651145 + 1482 WP_046100519.1 cardiolipin synthase -
A2I67_RS08475 (A2I67_08300) 1651170..1651817 + 648 WP_046100518.1 HD domain-containing protein -
A2I67_RS08480 (A2I67_08305) 1651908..1652420 - 513 WP_046100517.1 GNAT family N-acetyltransferase -
A2I67_RS08485 (A2I67_08310) 1652531..1653400 - 870 WP_015900837.1 membrane protein insertase YidC -
A2I67_RS08490 (A2I67_08315) 1653489..1654127 - 639 WP_046100516.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(27-65)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 69 a.a.        Molecular weight: 7833.72 Da        Isoelectric Point: 4.1353

>T63166 WP_015900832.1 NZ_CP015533:c1649194-1648988 [Staphylococcus carnosus]
MQNSVIKIDGMETEEEQHKLHQHLIEMTGVSAVQVDMDSNEVRISYETPVNLNNLEKEIYDAGYQILN

Download         Length: 207 bp

>T63166 NZ_CP015533:c1649194-1648988 [Staphylococcus carnosus]
ATGCAAAATAGTGTAATTAAAATTGATGGAATGGAAACTGAAGAAGAACAGCATAAATTGCATCAACATCTTATAGAAAT
GACAGGTGTATCTGCAGTGCAAGTGGATATGGATTCAAATGAAGTCAGAATCAGTTATGAAACACCTGTAAATCTCAATA
ATCTTGAAAAAGAAATCTATGATGCAGGTTATCAAATATTAAATTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11132.76 Da        Isoelectric Point: 6.4862

>AT63166 WP_015900833.1 NZ_CP015533:c1649499-1649206 [Staphylococcus carnosus]
MTENDKAHHSEQIKSNLKARLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAVRLLDHHMKGCIMHKVEDGHA
EEAMEELLVTVQKLIKD

Download         Length: 294 bp

>AT63166 NZ_CP015533:c1649499-1649206 [Staphylococcus carnosus]
ATGACCGAAAATGATAAAGCGCACCATTCAGAACAAATCAAATCCAACTTGAAAGCACGACTCAATCGAATTGAAGGACA
AGTCAGAGCAATTAACCGAATGATTGAAGAGGATGTATATTGTGATGATGTACTCACACAAATCCGTGCAACACGTTCAG
CTTTGAACAGTGTGGCAGTACGATTGCTTGATCATCACATGAAAGGTTGTATCATGCATAAAGTTGAGGATGGTCACGCT
GAAGAAGCGATGGAAGAATTACTTGTTACCGTTCAAAAATTGATTAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A380F432

Antitoxin

Source ID Structure
AlphaFold DB A0A380F6K8

References