Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1635625..1636139 Replicon chromosome
Accession NZ_CP015533
Organism Staphylococcus carnosus strain TMW 2.216

Toxin (Protein)


Gene name mazF Uniprot ID A0A3G4R0E2
Locus tag A2I67_RS08390 Protein ID WP_075140914.1
Coordinates 1635625..1635975 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID B9DMI8
Locus tag A2I67_RS08395 Protein ID WP_015900820.1
Coordinates 1635972..1636139 (-) Length 56 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A2I67_RS08370 (A2I67_08200) 1632652..1633422 - 771 WP_015900815.1 RNA polymerase sigma factor SigB -
A2I67_RS08375 (A2I67_08205) 1633397..1633876 - 480 WP_015900816.1 anti-sigma B factor RsbW -
A2I67_RS08380 (A2I67_08210) 1633878..1634204 - 327 WP_103211912.1 anti-sigma factor antagonist -
A2I67_RS08385 (A2I67_08215) 1634280..1635281 - 1002 WP_015900818.1 PP2C family protein-serine/threonine phosphatase -
A2I67_RS08390 (A2I67_08220) 1635625..1635975 - 351 WP_075140914.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
A2I67_RS08395 (A2I67_08225) 1635972..1636139 - 168 WP_015900820.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
A2I67_RS08400 (A2I67_08230) 1636220..1637374 - 1155 WP_046100526.1 alanine racemase -
A2I67_RS08405 (A2I67_08235) 1637438..1637791 - 354 WP_015900822.1 holo-ACP synthase -
A2I67_RS08410 (A2I67_08240) 1637793..1638293 - 501 WP_231635412.1 PH domain-containing protein -
A2I67_RS08415 (A2I67_08245) 1638283..1639770 - 1488 WP_103211911.1 PH domain-containing protein -
A2I67_RS08420 (A2I67_08250) 1639763..1640233 - 471 WP_015900825.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13075.18 Da        Isoelectric Point: 10.0708

>T63165 WP_075140914.1 NZ_CP015533:c1635975-1635625 [Staphylococcus carnosus]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIQISLGLTHRN

Download         Length: 351 bp

>T63165 NZ_CP015533:c1635975-1635625 [Staphylococcus carnosus]
ATGATGAGGAGAGGCGATGTTTACTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGGCCTGTTGT
AATTATACAAAATGACACTGGAAATAAGTATAGTCCGACAGTTATTGTGGCAGCAATTACTGGACGGATTAATAAAGCAA
AAATTCCTACTCATGTTGAAATTGAAAAAAGTAAATATAAATTGGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTCGATAAAAAGCGACTTAAAGAAAAACTGACATATTTATCAGACGAAAAGATGAAAGAAATTGATAATGCGATTCA
AATCAGCTTAGGCTTGACACATCGCAATTAA

Antitoxin


Download         Length: 56 a.a.        Molecular weight: 6211.75 Da        Isoelectric Point: 3.6836

>AT63165 WP_015900820.1 NZ_CP015533:c1636139-1635972 [Staphylococcus carnosus]
MSTFNQNRSYSLEQSLKEGYAQMADLNLSLATEAFSVECEACDCNESYLAFDKDE

Download         Length: 168 bp

>AT63165 NZ_CP015533:c1636139-1635972 [Staphylococcus carnosus]
ATGTCAACTTTTAATCAGAATAGAAGTTATAGTTTAGAACAGTCACTGAAAGAAGGCTATGCACAGATGGCTGACTTAAA
TCTCTCCCTCGCAACGGAGGCATTTTCAGTAGAATGTGAAGCCTGTGATTGCAATGAATCATATTTAGCTTTTGACAAGG
ATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G4R0E2

Antitoxin

Source ID Structure
AlphaFold DB A0A380F5Y4

References