Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1032188..1032702 Replicon chromosome
Accession NZ_CP015532
Organism Staphylococcus carnosus strain TMW 2.212

Toxin (Protein)


Gene name mazF Uniprot ID A0A3G4R0E2
Locus tag A2I65_RS04965 Protein ID WP_075140914.1
Coordinates 1032352..1032702 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID B9DMI8
Locus tag A2I65_RS04960 Protein ID WP_015900820.1
Coordinates 1032188..1032355 (+) Length 56 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A2I65_RS04935 1028337..1028564 + 228 WP_253720618.1 PH domain-containing protein -
A2I65_RS04940 (A2I65_04830) 1028557..1030044 + 1488 WP_015900824.1 PH domain-containing protein -
A2I65_RS04945 (A2I65_04835) 1030034..1030534 + 501 WP_231635412.1 PH domain-containing protein -
A2I65_RS04950 (A2I65_04840) 1030536..1030889 + 354 WP_015900822.1 holo-ACP synthase -
A2I65_RS04955 (A2I65_04845) 1030953..1032107 + 1155 WP_065865267.1 alanine racemase -
A2I65_RS04960 (A2I65_04850) 1032188..1032355 + 168 WP_015900820.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
A2I65_RS04965 (A2I65_04855) 1032352..1032702 + 351 WP_075140914.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
A2I65_RS04970 (A2I65_04860) 1033046..1034047 + 1002 WP_015900818.1 PP2C family protein-serine/threonine phosphatase -
A2I65_RS04975 (A2I65_04865) 1034123..1034449 + 327 WP_015900817.1 anti-sigma factor antagonist -
A2I65_RS04980 (A2I65_04870) 1034451..1034930 + 480 WP_015900816.1 anti-sigma B factor RsbW -
A2I65_RS04985 (A2I65_04875) 1034905..1035675 + 771 WP_253720566.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13075.18 Da        Isoelectric Point: 10.0708

>T63161 WP_075140914.1 NZ_CP015532:1032352-1032702 [Staphylococcus carnosus]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIQISLGLTHRN

Download         Length: 351 bp

>T63161 NZ_CP015532:1032352-1032702 [Staphylococcus carnosus]
ATGATGAGGAGAGGCGATGTTTACTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGGCCTGTTGT
AATTATACAAAATGACACTGGAAATAAGTATAGTCCGACAGTTATTGTGGCAGCAATTACTGGACGGATTAATAAAGCAA
AAATTCCTACTCATGTTGAAATTGAAAAAAGTAAATATAAATTGGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTCGATAAAAAGCGACTTAAAGAAAAACTGACATATTTATCAGACGAAAAGATGAAAGAAATTGATAATGCGATTCA
AATCAGCTTAGGCTTGACACATCGCAATTAA

Antitoxin


Download         Length: 56 a.a.        Molecular weight: 6211.75 Da        Isoelectric Point: 3.6836

>AT63161 WP_015900820.1 NZ_CP015532:1032188-1032355 [Staphylococcus carnosus]
MSTFNQNRSYSLEQSLKEGYAQMADLNLSLATEAFSVECEACDCNESYLAFDKDE

Download         Length: 168 bp

>AT63161 NZ_CP015532:1032188-1032355 [Staphylococcus carnosus]
ATGTCAACTTTTAATCAGAATAGAAGTTATAGTTTAGAACAGTCACTGAAAGAAGGCTATGCACAGATGGCTGACTTAAA
TCTCTCCCTAGCAACGGAGGCATTTTCAGTAGAATGTGAAGCCTGTGATTGCAATGAATCATATTTAGCTTTTGACAAGG
ACGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G4R0E2

Antitoxin

Source ID Structure
AlphaFold DB A0A380F5Y4

References