Detailed information of TA system TA063160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 1018829..1019340
• Replicon: chromosome
• Accession: NZ_CP015532
• Organism: Staphylococcus carnosus strain TMW 2.212

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: B9DMH5
• Locus tag: A2I65_RS04895
• Protein ID: WP_015900832.1
• Coordinates: 1019134..1019340 (+)
• Length: 69 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: -
• Locus tag: A2I65_RS04890
• Protein ID: WP_065865261.1
• Coordinates: 1018829..1019122 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2I65_RS04865 (A2I65_04760)	1014201..1014839	+	639	WP_015900838.1	thiamine phosphate synthase	-
A2I65_RS04870 (A2I65_04765)	1014928..1015797	+	870	WP_065865259.1	membrane protein insertase YidC	-
A2I65_RS04875 (A2I65_04770)	1015908..1016420	+	513	WP_015900836.1	GNAT family N-acetyltransferase	-
A2I65_RS04880 (A2I65_04775)	1016511..1017158	-	648	WP_253720562.1	HD domain-containing protein	-
A2I65_RS04885 (A2I65_04780)	1017183..1018664	-	1482	WP_253720563.1	cardiolipin synthase	-
A2I65_RS04890 (A2I65_04785)	1018829..1019122	+	294	WP_065865261.1	copper-sensing transcriptional repressor CsoR	Antitoxin
A2I65_RS04895 (A2I65_04790)	1019134..1019340	+	207	WP_015900832.1	heavy metal-associated domain-containing protein	Toxin
A2I65_RS04900 (A2I65_04795)	1019355..1019564	+	210	WP_015900831.1	copper ion binding protein	-
A2I65_RS04905 (A2I65_04800)	1019661..1021862	+	2202	WP_253720564.1	heavy metal translocating P-type ATPase	-
A2I65_RS04910	1021938..1022078	+	141	WP_167313208.1	Lmo0850 family protein	-
A2I65_RS04915 (A2I65_04805)	1022150..1023367	-	1218	WP_015900829.1	FtsW/RodA/SpoVE family cell cycle protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (27-65)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7833.72 Da
• Isoelectric Point: 4.1353

>T63160 WP_015900832.1 NZ_CP015532:1019134-1019340 [Staphylococcus carnosus]
MQNSVIKIDGMETEEEQHKLHQHLIEMTGVSAVQVDMDSNEVRISYETPVNLNNLEKEIYDAGYQILN

Nucleotide

• Length: 207 bp

>T63160 NZ_CP015532:1019134-1019340 [Staphylococcus carnosus]
ATGCAAAATAGTGTAATTAAAATTGATGGAATGGAAACTGAAGAAGAACAGCATAAATTGCATCAACATCTTATAGAAAT
GACAGGTGTATCTGCAGTGCAAGTGGATATGGATTCAAATGAAGTCAGAATCAGTTATGAAACACCTGTAAATCTCAATA
ATCTTGAAAAAGAAATCTATGATGCAGGTTATCAAATATTAAATTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11132.76 Da
• Isoelectric Point: 6.4862

>AT63160 WP_065865261.1 NZ_CP015532:1018829-1019122 [Staphylococcus carnosus]
MTENDKAHHSEQLKSNLKARLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAVRLLDHHMKGCIMHKVEDGHA
EEAMEELLVTVQKLIKD

Nucleotide

• Length: 294 bp

>AT63160 NZ_CP015532:1018829-1019122 [Staphylococcus carnosus]
ATGACCGAAAATGATAAAGCGCACCATTCAGAACAACTCAAATCCAACTTGAAAGCACGACTCAATCGAATTGAAGGACA
AGTCAGAGCAATTAACCGAATGATTGAAGAGGATGTATATTGTGATGATGTACTCACACAAATCCGTGCAACACGTTCAG
CTTTGAACAGTGTGGCAGTACGATTGCTTGATCATCACATGAAAGGTTGTATCATGCATAAAGTTGAGGATGGTCACGCT
GAAGAAGCGATGGAAGAATTGCTTGTTACCGTTCAAAAATTGATTAAAGATTAA

Structures

Toxin

• AlphaFold DB: A0A380F432