Detailed information of TA system TA063152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 254952..256528
• Replicon: plasmid unnamed
• Accession: NZ_CP015514
• Organism: Vibrio vulnificus strain FORC_036

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: FORC36_RS25615
• Protein ID: WP_085762241.1
• Coordinates: 254952..256277 (-)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: FORC36_RS25620
• Protein ID: WP_045592353.1
• Coordinates: 256271..256528 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC36_RS25570 (FORC36_4900)	250262..250570	-	309	WP_223371017.1	hypothetical protein	-
FORC36_RS25575 (FORC36_4901)	250579..250803	-	225	WP_021485441.1	hypothetical protein	-
FORC36_RS25580 (FORC36_4902)	251002..251598	+	597	WP_021485939.1	hypothetical protein	-
FORC36_RS25585 (FORC36_4903)	251698..251982	+	285	WP_045592340.1	hypothetical protein	-
FORC36_RS25590 (FORC36_4904)	252036..252698	+	663	WP_085762237.1	DUF262 domain-containing protein	-
FORC36_RS25595 (FORC36_4905)	252797..253201	+	405	WP_085762238.1	hypothetical protein	-
FORC36_RS25600 (FORC36_4906)	253235..253498	+	264	WP_045592346.1	hypothetical protein	-
FORC36_RS25605 (FORC36_4907)	253673..254116	+	444	WP_085762239.1	EF-hand domain-containing protein	-
FORC36_RS25610 (FORC36_4908)	254230..254916	+	687	WP_223371018.1	hypothetical protein	-
FORC36_RS25615 (FORC36_4909)	254952..256277	-	1326	WP_085762241.1	type II toxin-antitoxin system HipA family toxin	Toxin
FORC36_RS25620 (FORC36_4910)	256271..256528	-	258	WP_045592353.1	helix-turn-helix domain-containing protein	Antitoxin
FORC36_RS25625 (FORC36_4911)	256765..257610	-	846	WP_085762242.1	hypothetical protein	-
FORC36_RS25630 (FORC36_4912)	257745..258617	+	873	WP_085762243.1	ATPase, T2SS/T4P/T4SS family	-
FORC36_RS25635 (FORC36_4913)	258607..259059	+	453	WP_053319301.1	hypothetical protein	-
FORC36_RS25640 (FORC36_4914)	259083..259487	-	405	WP_085762244.1	hypothetical protein	-
FORC36_RS25645 (FORC36_4915)	259543..260550	-	1008	WP_085762245.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..925374	925374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-64)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 49454.74 Da
• Isoelectric Point: 6.7016

>T63152 WP_085762241.1 NZ_CP015514:c256277-254952 [Vibrio vulnificus]
MVKQLRVLMNGESVGVFTKEPSGAHSFQYGEEWLNSSKSRPISLSIPLRREPYRGDIVINFFDNLLPDNPQVRDRIVARY
GAKTKQPFDLLEKIGRDSVGAITLIPEGEPIPEFKGIRATELSLIKLEQVLNSYADNIPLGMVDGNDDFRISIAGAQEKT
ALLKLDERWFIPQGATPTTHIIKLPIGEIRTQDSVLDLTTSVDNELVCMRLSEAFGLPTASCDVIRAGGVRALAVERFDR
RFSQLKDWIVRLPQEDFCQVSGMPSASKYESDGGIGIEGIMDHLLGSEVPQEDRRTFMRSQVLFYLLAAIDGHSKNFSLF
IEPHGKYRLTPLYDIMSAYPLMGGRGLHERKIKLAMGLKASSGYKRKWHQIQPRHFIGTARLVKYSSDSMRDILDEFIAD
CGQVIDLVGNNLPDDINENVRDKIFEGLTKSVKKLSLGLND

Nucleotide

• Length: 1326 bp

>T63152 NZ_CP015514:c256277-254952 [Vibrio vulnificus]
ATGGTAAAGCAATTACGCGTGTTAATGAATGGTGAGTCGGTAGGGGTGTTCACTAAAGAACCTAGTGGTGCTCATAGCTT
TCAATATGGCGAAGAATGGTTAAACTCTAGTAAATCTCGCCCGATATCATTATCAATCCCCCTTCGCCGTGAGCCGTACA
GGGGTGATATAGTGATTAACTTTTTCGACAACCTGCTTCCAGATAACCCTCAGGTTCGAGACCGAATCGTTGCTCGATAC
GGTGCCAAAACAAAACAGCCATTCGATCTTTTGGAAAAGATAGGGCGCGATAGTGTTGGGGCAATCACCCTTATTCCTGA
AGGCGAACCGATTCCAGAATTTAAGGGGATAAGAGCTACTGAGCTTTCCCTTATAAAGCTTGAACAAGTTCTTAATTCCT
ATGCAGACAATATCCCTTTAGGCATGGTTGATGGAAATGATGATTTCAGGATCTCCATTGCTGGCGCTCAAGAGAAAACT
GCCTTGTTGAAGTTGGATGAGCGTTGGTTTATACCGCAAGGTGCAACTCCAACCACACACATTATAAAACTTCCGATTGG
TGAAATACGGACGCAGGACAGCGTTTTAGACCTAACAACCAGTGTTGATAATGAACTCGTGTGTATGAGGCTTTCTGAAG
CGTTTGGACTGCCTACAGCCTCTTGTGACGTTATTAGGGCTGGTGGAGTAAGGGCGCTTGCCGTTGAGCGTTTCGATAGA
CGATTCTCACAACTAAAAGATTGGATTGTACGCTTGCCTCAAGAAGACTTTTGCCAGGTGTCTGGTATGCCATCAGCAAG
CAAATATGAATCAGATGGTGGTATCGGTATTGAAGGAATAATGGATCATCTTCTTGGCTCAGAGGTTCCTCAGGAAGACA
GGAGAACCTTTATGCGTTCTCAAGTTCTCTTCTATTTACTTGCTGCAATAGATGGTCACTCAAAAAACTTTTCGCTATTC
ATAGAGCCTCATGGTAAGTACCGATTAACGCCTTTGTACGACATTATGTCAGCCTACCCTCTTATGGGTGGTAGAGGGCT
TCATGAAAGAAAAATCAAGCTGGCAATGGGACTAAAGGCAAGTAGCGGATACAAACGTAAGTGGCATCAGATACAGCCTA
GACATTTTATCGGTACAGCGAGGCTGGTGAAGTATTCATCGGACTCTATGAGAGATATTCTGGATGAGTTTATTGCTGAT
TGTGGACAGGTTATCGATCTAGTTGGGAACAATCTTCCAGATGATATCAATGAGAATGTTCGGGATAAAATCTTTGAGGG
ATTAACAAAATCAGTGAAAAAACTGAGTCTTGGTCTGAATGATTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9676.04 Da
• Isoelectric Point: 8.7273

>AT63152 WP_045592353.1 NZ_CP015514:c256528-256271 [Vibrio vulnificus]
MLITSSRQLAIHLRDERKSRKLSQAKVADPVGIKQDTVSKFEVNPETTSIQTLFRILSSLNLEMHIVPKGQSIDPKTDNE
SDEKW

Nucleotide

• Length: 258 bp

>AT63152 NZ_CP015514:c256528-256271 [Vibrio vulnificus]
ATGTTGATAACATCATCCAGGCAGTTAGCAATACATCTTCGTGATGAAAGAAAAAGCAGGAAGCTATCTCAAGCAAAAGT
TGCCGATCCAGTAGGGATAAAACAAGACACGGTTTCTAAGTTTGAGGTTAATCCTGAAACCACCAGCATTCAAACGCTAT
TTCGAATCTTATCATCGTTAAATTTGGAAATGCATATTGTGCCGAAAGGTCAAAGCATCGACCCAAAAACTGATAATGAG
AGTGATGAGAAATGGTAA