Detailed information of TA system TA063150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 356182..356741
• Replicon: chromosome
• Accession: NZ_CP015513
• Organism: Vibrio vulnificus strain FORC_036

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FORC36_RS17255
• Protein ID: WP_072610555.1
• Coordinates: 356463..356741 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A3Q0L0M2
• Locus tag: FORC36_RS17250
• Protein ID: WP_011082348.1
• Coordinates: 356182..356466 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC36_RS17220 (FORC36_3213)	351312..352505	+	1194	WP_011082341.1	glycine C-acetyltransferase	-
FORC36_RS17225 (FORC36_3214)	352550..353581	+	1032	WP_011082342.1	L-threonine 3-dehydrogenase	-
FORC36_RS17230 (FORC36_3215)	353661..354161	-	501	WP_017420104.1	opioid growth factor receptor-related protein	-
FORC36_RS17235 (FORC36_3216)	354239..354502	-	264	WP_011082346.1	hypothetical protein	-
FORC36_RS17240 (FORC36_3217)	354688..355221	-	534	WP_085761582.1	hypothetical protein	-
FORC36_RS17245 (FORC36_3218)	355356..355964	-	609	WP_011151714.1	hypothetical protein	-
FORC36_RS17250 (FORC36_3219)	356182..356466	+	285	WP_011082348.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
FORC36_RS17255 (FORC36_3220)	356463..356741	+	279	WP_072610555.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
FORC36_RS17260	356770..356886	-	117	WP_085762040.1	DUF3265 domain-containing protein	-
FORC36_RS17265 (FORC36_3221)	356887..358623	-	1737	WP_085761583.1	ATP-binding protein	-
FORC36_RS17270 (FORC36_3222)	359311..359898	-	588	WP_011151719.1	DUF1471 domain-containing protein	-
FORC36_RS17275 (FORC36_3223)	361214..361729	-	516	WP_085761584.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	351312..359898	8586

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-45)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10888.57 Da
• Isoelectric Point: 4.3423

>T63150 WP_072610555.1 NZ_CP015513:356463-356741 [Vibrio vulnificus]
MILWEEESLNDREKIFEFLYDFNLDAAEKTDNLIEAKVENLLEQPLMGVQRDGIHGRLLIIPEISMVISYWVEGDIIRVM
RVLHQKQKFPTD

Nucleotide

• Length: 279 bp

>T63150 NZ_CP015513:356463-356741 [Vibrio vulnificus]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTCGAGTTTCTCTATGACTTCAACCTTGATGCGGC
AGAAAAAACTGACAATCTCATTGAAGCAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTACAACGAGATGGCA
TCCACGGTCGATTACTCATTATTCCTGAGATTTCGATGGTTATCTCTTACTGGGTCGAGGGCGATATCATCCGAGTTATG
CGTGTCCTCCACCAGAAACAAAAATTCCCTACGGATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 11083.43 Da
• Isoelectric Point: 9.2167

>AT63150 WP_011082348.1 NZ_CP015513:356182-356466 [Vibrio vulnificus]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKTLSHDAWLTEQVNLAFEKFDSGKSVFIEHQTAKSR
MEERKARIRNRGKQ

Nucleotide

• Length: 285 bp

>AT63150 NZ_CP015513:356182-356466 [Vibrio vulnificus]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAGAGCCAAGGTCGCAC
TCTTAGTGACGCATGCCGTGAACTTACTGAGCAACTCGCTGAACAACAAAGAAAAACATTGTCTCACGATGCATGGCTAA
CTGAACAAGTAAATCTAGCATTTGAGAAGTTTGACTCAGGAAAATCCGTTTTCATTGAGCACCAAACTGCTAAATCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCGAGGTAAGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A3Q0L0M2