Detailed information of TA system TA063142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 55475..56057
• Replicon: chromosome
• Accession: NZ_CP015511
• Organism: Brevundimonas sp. GW460-12-10-14-LB2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: A4249_RS00325
• Protein ID: WP_066549794.1
• Coordinates: 55475..55795 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A168MX47
• Locus tag: A4249_RS00330
• Protein ID: WP_066549799.1
• Coordinates: 55818..56057 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4249_RS00300	51179..51907	+	729	WP_066549781.1	hydroxyacylglutathione hydrolase	-
A4249_RS00305	51904..52671	-	768	WP_066549784.1	DUF4908 domain-containing protein	-
A4249_RS00310	52721..53446	-	726	WP_066549787.1	acetoacetyl-CoA reductase	-
A4249_RS00315	53590..54645	-	1056	WP_174085380.1	NAD(P)-dependent alcohol dehydrogenase	-
A4249_RS00320	54729..55478	-	750	WP_066549792.1	DUF72 domain-containing protein	-
A4249_RS00325	55475..55795	-	321	WP_066549794.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A4249_RS00330	55818..56057	-	240	WP_066549799.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
A4249_RS00335	56112..57683	-	1572	WP_066549803.1	response regulator	-
A4249_RS16420	57796..58182	+	387	WP_156494554.1	hypothetical protein	-
A4249_RS00340	58095..58532	+	438	WP_066549806.1	hypothetical protein	-
A4249_RS00345	58529..59890	-	1362	WP_066549809.1	glutamate--cysteine ligase	-
A4249_RS00350	60025..60756	-	732	WP_066549811.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
A4249_RS00355	60753..61013	-	261	WP_066549813.1	Txe/YoeB family addiction module toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (1-77)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12034.72 Da
• Isoelectric Point: 8.5378

>T63142 WP_066549794.1 NZ_CP015511:c55795-55475 [Brevundimonas sp. GW460-12-10-14-LB2]
MAKLRVSKAARTDMRAIFRQSVEMFGLSQADAYVAALHHALDLIATYPKSNRERAETAPPVRVHPYKAHVIVYAIDDQGV
LVLRIRHTREDWTADPIRDREAGDEP

Nucleotide

• Length: 321 bp

>T63142 NZ_CP015511:c55795-55475 [Brevundimonas sp. GW460-12-10-14-LB2]
TTGGCCAAGCTGCGGGTATCCAAGGCCGCGCGAACCGATATGCGTGCGATCTTTCGACAGAGCGTCGAGATGTTTGGGTT
GAGCCAGGCTGACGCCTACGTCGCCGCTCTTCACCACGCGCTCGATCTCATCGCGACATATCCCAAGTCGAACCGGGAAA
GAGCAGAGACCGCGCCGCCTGTGCGAGTTCATCCTTACAAGGCGCACGTCATCGTCTATGCCATCGACGATCAGGGCGTT
CTGGTGCTCCGCATCCGACATACGCGAGAGGACTGGACAGCCGATCCCATCCGAGACCGCGAAGCCGGAGACGAACCATG
A

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9046.23 Da
• Isoelectric Point: 4.6151

>AT63142 WP_066549799.1 NZ_CP015511:c56057-55818 [Brevundimonas sp. GW460-12-10-14-LB2]
MATMNISLPDPMKAWVEEQAKSGRYANTSDVVRDLIRREQVKAEKIANMQRLIDEAYASGISDQTPREIFEEVRAEYLA

Nucleotide

• Length: 240 bp

>AT63142 NZ_CP015511:c56057-55818 [Brevundimonas sp. GW460-12-10-14-LB2]
ATGGCGACGATGAACATCTCCCTGCCCGATCCAATGAAGGCCTGGGTCGAGGAACAGGCGAAATCCGGCCGCTACGCCAA
CACCTCGGACGTGGTGCGCGACCTGATCCGCCGCGAGCAGGTCAAGGCAGAGAAGATCGCCAACATGCAAAGGTTGATCG
ACGAAGCCTATGCATCAGGCATCAGCGACCAAACGCCACGAGAAATTTTCGAAGAGGTTCGCGCCGAATATCTGGCCTAA

Structures

Antitoxin

• AlphaFold DB: A0A168MX47