Detailed information of TA system TA063141

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 103404..104205
• Replicon: plasmid pBO1
• Accession: NZ_CP015507
• Organism: Bacillus oceanisediminis 2691

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: A0A160MHJ4
• Locus tag: A361_RS27665
• Protein ID: WP_019380940.1
• Coordinates: 103404..103808 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: A0A160MJA0
• Locus tag: A361_RS27670
• Protein ID: WP_019380939.1
• Coordinates: 103798..104205 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A361_RS27640	98768..99139	+	372	WP_019380943.1	hypothetical protein	-
A361_RS27645	99432..100628	-	1197	Protein_102	IS110 family transposase	-
A361_RS27655	101093..101686	+	594	WP_019380942.1	DUF4433 domain-containing protein	-
A361_RS27660	101719..102759	+	1041	WP_019380941.1	macro domain-containing protein	-
A361_RS27665	103404..103808	-	405	WP_019380940.1	DUF86 domain-containing protein	Toxin
A361_RS27670	103798..104205	-	408	WP_019380939.1	nucleotidyltransferase domain-containing protein	Antitoxin
A361_RS27675	104697..105032	-	336	WP_019380938.1	(2Fe-2S) ferredoxin domain-containing protein	-
A361_RS29190	105633..105872	-	240	WP_081757328.1	glutaredoxin family protein	-
A361_RS27680	106246..106649	-	404	Protein_109	DUF86 domain-containing protein	-
A361_RS27685	106639..107046	-	408	WP_019380934.1	nucleotidyltransferase domain-containing protein	-
A361_RS27690	107239..107751	-	513	WP_019380933.1	DUF2269 family protein	-
A361_RS27695	107748..107969	-	222	WP_019380932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..386866	386866

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (14-131)

Antitoxin

• Polymerase beta, nucleotidyltransferase (7-98)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15480.04 Da
• Isoelectric Point: 6.6378

>T63141 WP_019380940.1 NZ_CP015507:c103808-103404 [Bacillus oceanisediminis 2691]
MKSDVILNKINIIERCIKRIHEEYDQNPKNLENYTKQDSIILNLQRACEASIDLAMHIVAVKKLGLPQQSRDAFTLLEAE
GIIPSSLSDKMKAMVGFRNIAVHDYQEINLVILQKILDNHLVDFFEYTKTILMH

Nucleotide

• Length: 405 bp

>T63141 NZ_CP015507:c103808-103404 [Bacillus oceanisediminis 2691]
ATGAAAAGTGATGTAATTTTAAATAAAATAAATATAATTGAACGCTGTATTAAACGAATTCACGAGGAATACGACCAGAA
TCCTAAAAATCTAGAGAATTATACAAAGCAAGATTCAATTATTTTAAATCTGCAGCGGGCTTGTGAGGCAAGTATTGACT
TGGCCATGCACATAGTAGCCGTAAAAAAACTGGGACTCCCACAACAAAGCCGGGATGCATTTACCTTATTGGAAGCTGAG
GGTATTATTCCCTCCTCTCTTTCAGATAAGATGAAAGCTATGGTTGGATTTCGTAATATTGCAGTTCATGATTACCAAGA
GATTAATTTAGTGATCCTTCAAAAGATACTTGATAATCATTTAGTGGATTTCTTTGAATATACAAAAACCATTTTAATGC
ACTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15464.67 Da
• Isoelectric Point: 4.7024

>AT63141 WP_019380939.1 NZ_CP015507:c104205-103798 [Bacillus oceanisediminis 2691]
MNPHLEETIIDVLREKLSPDWIVMFGSTVSGNTHKESDIDIAYLSDTIKADKYQIFMIAQELAAKLNKDVDLIDLNQAST
VFQAQIVQSGTTIYCTNERKKAEFEVKVLKMYTKLNEERSEILKKIDESGTIYEK

Nucleotide

• Length: 408 bp

>AT63141 NZ_CP015507:c104205-103798 [Bacillus oceanisediminis 2691]
TTGAATCCACACCTTGAAGAAACCATTATAGATGTACTTCGTGAAAAATTATCCCCCGATTGGATAGTTATGTTTGGTTC
AACCGTTAGTGGAAATACCCATAAAGAAAGTGACATTGATATTGCATATTTGAGTGACACAATTAAAGCAGATAAATATC
AAATATTTATGATTGCTCAGGAATTAGCAGCAAAACTCAACAAAGATGTGGACCTCATTGATTTAAATCAGGCCAGTACA
GTATTTCAAGCCCAAATAGTTCAATCCGGAACAACTATTTATTGTACCAATGAGCGAAAAAAAGCTGAATTTGAAGTTAA
AGTATTAAAAATGTATACGAAGCTAAATGAGGAGCGATCAGAGATATTAAAAAAAATTGACGAAAGCGGGACAATATATG
AAAAGTGA

Structures

Toxin

• AlphaFold DB: A0A160MHJ4

Antitoxin

• AlphaFold DB: A0A160MJA0