Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 34057..34580 Replicon plasmid unnamed 2
Accession NZ_CP015502
Organism Klebsiella pneumoniae strain SKGH01

Toxin (Protein)


Gene name ccdB Uniprot ID V5RNQ6
Locus tag A7B01_RS28700 Protein ID WP_015632464.1
Coordinates 34275..34580 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V5RQR8
Locus tag A7B01_RS28695 Protein ID WP_015632465.1
Coordinates 34057..34275 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7B01_RS28675 30200..31471 - 1272 WP_016162068.1 Y-family DNA polymerase -
A7B01_RS28680 31471..31902 - 432 WP_015632467.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
A7B01_RS28685 32066..32551 - 486 Protein_32 IS5 family transposase -
A7B01_RS28690 32846..33871 - 1026 WP_015632466.1 IS110 family transposase -
A7B01_RS28695 34057..34275 + 219 WP_015632465.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A7B01_RS28700 34275..34580 + 306 WP_015632464.1 type II toxin-antitoxin system toxin CcdB Toxin
A7B01_RS28705 34725..35069 + 345 WP_015632463.1 hypothetical protein -
A7B01_RS31765 35183..35677 + 495 WP_161537194.1 IS1 family transposase -
A7B01_RS28710 35881..36138 - 258 WP_016162071.1 helix-turn-helix domain-containing protein -
A7B01_RS28715 36568..36795 + 228 WP_016162072.1 hypothetical protein -
A7B01_RS28720 36809..36931 + 123 Protein_40 transcriptional regulator -
A7B01_RS28725 37137..37580 - 444 WP_015632459.1 type II toxin-antitoxin system HicB family antitoxin -
A7B01_RS31770 37606..37788 - 183 WP_015632458.1 type II toxin-antitoxin system HicA family toxin -
A7B01_RS28730 38149..39129 + 981 WP_015632457.1 partitioning protein -
A7B01_RS28735 39122..39550 + 429 WP_015632456.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / qnrB1 - 1..140874 140874


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11361.90 Da        Isoelectric Point: 5.5252

>T63135 WP_015632464.1 NZ_CP015502:34275-34580 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Download         Length: 306 bp

>T63135 NZ_CP015502:34275-34580 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8194.13 Da        Isoelectric Point: 6.2224

>AT63135 WP_015632465.1 NZ_CP015502:34057-34275 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Download         Length: 219 bp

>AT63135 NZ_CP015502:34057-34275 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0ND19

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0L1T7

References