Detailed information of TA system TA063133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 13675..14585
• Replicon: plasmid unnamed 1
• Accession: NZ_CP015501
• Organism: Klebsiella pneumoniae strain SKGH01

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2N4Z2R0
• Locus tag: A7B01_RS27315
• Protein ID: WP_004197882.1
• Coordinates: 14115..14585 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: A7B01_RS27310
• Protein ID: WP_004181895.1
• Coordinates: 13675..14118 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7B01_RS31495	8840..9055	-	216	Protein_9	IS200/IS605 family transposase	-
A7B01_RS27290	9057..9626	+	570	Protein_10	IS200/IS605 family element transposase accessory protein TnpB	-
A7B01_RS27295	10315..11798	+	1484	Protein_11	group II intron reverse transcriptase/maturase	-
A7B01_RS27300	11881..12303	+	423	WP_004197884.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
A7B01_RS27305	12303..13574	+	1272	WP_004197886.1	translesion error-prone DNA polymerase V subunit UmuC	-
A7B01_RS27310	13675..14118	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
A7B01_RS27315	14115..14585	+	471	WP_004197882.1	RES family NAD+ phosphorylase	Toxin
A7B01_RS27320	14695..14955	-	261	WP_004026352.1	hypothetical protein	-
A7B01_RS27325	15193..16254	-	1062	WP_060591524.1	IS481 family transposase	-
A7B01_RS27330	16381..17913	-	1533	WP_004118819.1	IS3 family transposase	-
A7B01_RS27335	18002..19342	+	1341	WP_004196353.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..281190	281190

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17633.92 Da
• Isoelectric Point: 4.5152

>T63133 WP_004197882.1 NZ_CP015501:14115-14585 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDFFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T63133 NZ_CP015501:14115-14585 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTTTTTTACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCTCTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATTGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT63133 WP_004181895.1 NZ_CP015501:13675-14118 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT63133 NZ_CP015501:13675-14118 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A2N4Z2R0

Antitoxin

• AlphaFold DB: A0A2J4RLY9