Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1299040..1299776 Replicon chromosome
Accession NZ_CP015500
Organism Klebsiella pneumoniae strain SKGH01

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag A7B01_RS06630 Protein ID WP_032433360.1
Coordinates 1299294..1299776 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag A7B01_RS06625 Protein ID WP_003026799.1
Coordinates 1299040..1299306 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7B01_RS06600 1294686..1295825 + 1140 WP_032433368.1 mannitol dehydrogenase -
A7B01_RS06605 1295854..1296516 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
A7B01_RS06610 1296500..1297504 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
A7B01_RS06615 1297522..1298154 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
A7B01_RS06620 1298164..1298727 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
A7B01_RS06625 1299040..1299306 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
A7B01_RS06630 1299294..1299776 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
A7B01_RS06635 1300137..1300736 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
A7B01_RS06640 1300949..1301893 - 945 WP_077254249.1 fimbrial protein -
A7B01_RS06645 1301905..1302483 - 579 WP_032432061.1 type 1 fimbrial protein -
A7B01_RS06650 1302487..1303227 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1269263..1310719 41456


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T63118 WP_032433360.1 NZ_CP015500:1299294-1299776 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T63118 NZ_CP015500:1299294-1299776 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT63118 WP_003026799.1 NZ_CP015500:1299040-1299306 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT63118 NZ_CP015500:1299040-1299306 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References