Detailed information of TA system TA063113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 32574..33175
• Replicon: plasmid pFAM8105
• Accession: NZ_CP015497
• Organism: Lactobacillus helveticus strain FAM8105

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: U6FEM0
• Locus tag: Lh8105_RS11770
• Protein ID: WP_011241744.1
• Coordinates: 32831..33175 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: U6FC11
• Locus tag: Lh8105_RS11765
• Protein ID: WP_023192325.1
• Coordinates: 32574..32837 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Lh8105_RS11735	28185..29777	-	1593	WP_035516257.1	ABC transporter ATP-binding protein/permease	-
Lh8105_RS11745	30092..31270	+	1179	WP_061287174.1	IS256 family transposase	-
Lh8105_RS11755	31566..32150	-	585	WP_101854100.1	recombinase family protein	-
Lh8105_RS11760	32254..32478	-	225	Protein_28	hypothetical protein	-
Lh8105_RS11765	32574..32837	+	264	WP_023192325.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Lh8105_RS11770	32831..33175	+	345	WP_011241744.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Lh8105_RS11780	33607..34047	+	441	WP_023192327.1	hypothetical protein	-
Lh8105_RS11785	34063..34578	+	516	WP_023192328.1	hypothetical protein	-
Lh8105_RS11790	34571..34987	+	417	WP_023192329.1	hypothetical protein	-
Lh8105_RS11795	35314..35418	+	105	WP_016378676.1	putative holin-like toxin	-
Lh8105_RS11800	35561..36676	-	1116	WP_101854101.1	hypothetical protein	-
Lh8105_RS11805	36679..36894	-	216	WP_003713050.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..45858	45858
	-	flank	IS/Tn	-	-	30092..31270	1178

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-101)

Antitoxin

• Antitoxin PemI-like (8-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13010.79 Da
• Isoelectric Point: 5.5556

>T63113 WP_011241744.1 NZ_CP015497:32831-33175 [Lactobacillus helveticus]
MVSQGDIFYVNFNPSRGHEQMNRRPAIALSNDLVCQTSNMTIVAPISSTERNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVADEAIVDHVSREELEEIITLYKLLFSIDDK

Nucleotide

• Length: 345 bp

>T63113 NZ_CP015497:32831-33175 [Lactobacillus helveticus]
ATGGTAAGTCAGGGCGATATATTCTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAGGCGGCCAGCCAT
TGCTCTAAGCAATGATCTAGTCTGCCAAACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCGAGCGGAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTGTTAGATCAAACAATAGCTTTAGATTTGCGG
GCAAGACATGTTGCTGACGAAGCTATTGTCGATCATGTATCGCGTGAAGAATTAGAAGAAATAATCACTCTGTACAAATT
ATTGTTTAGTATTGATGACAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10169.52 Da
• Isoelectric Point: 6.4708

>AT63113 WP_023192325.1 NZ_CP015497:32574-32837 [Lactobacillus helveticus]
MELIKEQTRLAKWGNSKAARIPSQIIRKLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Nucleotide

• Length: 264 bp

>AT63113 NZ_CP015497:32574-32837 [Lactobacillus helveticus]
ATGGAACTTATTAAAGAACAAACACGCCTGGCAAAGTGGGGCAACTCGAAAGCTGCTAGAATTCCTAGCCAGATTATTAG
AAAATTGAAATTAGATGATAACCAAGATATGACAATAACAATTGAAAATGGATCGATTGTTTTAACACCAATAAAAAAGA
ATCCGACTAATATTCACGAACTATTTAAGGACTGGCAAGATGACGGGAAACGTGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Structures

Toxin

• AlphaFold DB: U6FEM0

Antitoxin

• AlphaFold DB: U6FC11