Detailed information of TA system TA063098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-YefM
• Location: 17211..17727
• Replicon: plasmid unnamed1
• Accession: NZ_CP015450
• Organism: Dietzia lutea strain YIM 80766

Toxin (Protein)

• Gene name: relB
• Uniprot ID: A0A166Q8Z2
• Locus tag: A6035_RS17490
• Protein ID: WP_061228632.1
• Coordinates: 17211..17468 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: A6035_RS17495
• Protein ID: WP_108849392.1
• Coordinates: 17470..17727 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6035_RS17470	13105..14247	-	1143	WP_108849389.1	CoA transferase	-
A6035_RS17475	14339..15994	-	1656	WP_159149251.1	gamma-glutamyltransferase family protein	-
A6035_RS17480	16105..16266	+	162	Protein_16	IS3 family transposase	-
A6035_RS17485	16743..17075	-	333	WP_108849391.1	Lsr2 family protein	-
A6035_RS17490	17211..17468	-	258	WP_061228632.1	Txe/YoeB family addiction module toxin	Toxin
A6035_RS17495	17470..17727	-	258	WP_108849392.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
A6035_RS17500	17789..19192	-	1404	WP_108849393.1	amidase	-
A6035_RS17505	19740..20156	-	417	WP_108849417.1	hypothetical protein	-
A6035_RS17510	20311..20607	-	297	WP_108849394.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..67767	67767
	-	flank	IS/Tn	-	-	16090..16269	179

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10355.73 Da
• Isoelectric Point: 8.0063

>T63098 WP_061228632.1 NZ_CP015450:c17468-17211 [Dietzia lutea]
VLLVWDDNAWQDYLWWQQQDRKILKRINLLLQDITRNGNEGIGKPEPLKYDFAGYWSRRITDEHRLVYKVTDTEVRIATC
RYHYG

Nucleotide

• Length: 258 bp

>T63098 NZ_CP015450:c17468-17211 [Dietzia lutea]
GTGCTGCTCGTCTGGGACGACAACGCCTGGCAGGACTACCTCTGGTGGCAACAACAGGACCGCAAGATCCTCAAACGCAT
CAACCTGCTCCTGCAAGACATCACCCGCAACGGCAATGAAGGCATCGGCAAACCCGAACCCCTCAAATACGACTTCGCCG
GCTACTGGTCCAGACGCATTACCGACGAGCACCGACTCGTCTACAAAGTCACCGACACGGAAGTCCGTATCGCCACCTGC
CGCTACCACTACGGGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9710.84 Da
• Isoelectric Point: 4.5716

>AT63098 WP_108849392.1 NZ_CP015450:c17727-17470 [Dietzia lutea]
MKTMSYTESRARYAEVLDGVTNDREEVIITRAGHEPVVMVSLDDYESLRETAHLMRSPANARRLLDAMERLESGRGQARD
LLDED

Nucleotide

• Length: 258 bp

>AT63098 NZ_CP015450:c17727-17470 [Dietzia lutea]
ATGAAAACCATGAGCTACACCGAGTCCCGGGCCCGCTACGCCGAAGTCCTCGACGGGGTCACCAACGACCGCGAGGAAGT
CATCATCACCCGCGCCGGTCACGAACCGGTGGTGATGGTCTCCCTCGACGACTACGAGTCGCTGCGCGAGACCGCCCACC
TCATGCGCTCGCCAGCCAACGCGCGCAGGCTTCTCGACGCGATGGAACGTCTCGAGTCCGGCCGCGGCCAGGCGCGCGAC
CTGCTCGACGAAGACTGA

Structures

Toxin

• AlphaFold DB: A0A166Q8Z2