Detailed information of TA system TA063075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1499452..1500089
• Replicon: chromosome
• Accession: NZ_CP015443
• Organism: Bacillus velezensis strain CC09

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: A1D33_RS06800
• Protein ID: WP_003156187.1
• Coordinates: 1499452..1499802 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: A1D33_RS06805
• Protein ID: WP_003156188.1
• Coordinates: 1499808..1500089 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A1D33_RS06760	1494492..1495094	-	603	WP_039252641.1	SpoIIE family protein phosphatase	-
A1D33_RS06765	1495094..1495882	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
A1D33_RS06770	1495848..1496330	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
A1D33_RS06775	1496327..1496656	-	330	WP_031378636.1	anti-sigma factor antagonist	-
A1D33_RS06780	1496720..1497727	-	1008	WP_039252645.1	PP2C family protein-serine/threonine phosphatase	-
A1D33_RS06785	1497739..1498140	-	402	WP_003156178.1	anti-sigma regulatory factor	-
A1D33_RS06790	1498143..1498508	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
A1D33_RS06795	1498513..1499334	-	822	WP_062623254.1	STAS domain-containing protein	-
A1D33_RS06800	1499452..1499802	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
A1D33_RS06805	1499808..1500089	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
A1D33_RS06810	1500209..1501378	-	1170	WP_039252648.1	alanine racemase	-
A1D33_RS06815	1501495..1502502	-	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
A1D33_RS06820	1502667..1503032	-	366	WP_062623253.1	holo-ACP synthase	-
A1D33_RS06825	1503125..1503724	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T63075 WP_003156187.1 NZ_CP015443:c1499802-1499452 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T63075 NZ_CP015443:c1499802-1499452 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT63075 WP_003156188.1 NZ_CP015443:c1500089-1499808 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT63075 NZ_CP015443:c1500089-1499808 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5