Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2532413..2533107 Replicon chromosome
Accession NZ_CP015436
Organism Anoxybacillus sp. B7M1

Toxin (Protein)


Gene name mazF Uniprot ID A0A160FBF3
Locus tag GFC29_RS12200 Protein ID WP_044744379.1
Coordinates 2532413..2532799 (-) Length 129 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A160FBE5
Locus tag GFC29_RS12205 Protein ID WP_044744380.1
Coordinates 2532799..2533107 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFC29_RS12170 2528053..2529543 + 1491 WP_044744375.1 glycerol kinase GlpK -
GFC29_RS12175 2529536..2529892 - 357 WP_044744376.1 hypothetical protein -
GFC29_RS12180 2530000..2530638 + 639 WP_044744377.1 Bax inhibitor-1/YccA family protein -
GFC29_RS12185 2530732..2531346 + 615 WP_172798625.1 hypothetical protein -
GFC29_RS19885 2531405..2531626 + 222 Protein_2423 transposase -
GFC29_RS12190 2531716..2532038 - 323 Protein_2424 hypothetical protein -
GFC29_RS12195 2532035..2532370 - 336 Protein_2425 hypothetical protein -
GFC29_RS12200 2532413..2532799 - 387 WP_044744379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GFC29_RS12205 2532799..2533107 - 309 WP_044744380.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GFC29_RS19195 2533607..2534200 - 594 Protein_2428 pre-toxin TG domain-containing protein -
GFC29_RS19200 2534748..2534840 - 93 Protein_2429 zinc-ribbon domain-containing protein -
GFC29_RS19205 2534927..2535296 + 370 Protein_2430 hypothetical protein -
GFC29_RS19210 2535726..2535941 - 216 WP_156487540.1 ATP-binding protein -
GFC29_RS12225 2536506..2536991 - 486 Protein_2432 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
GFC29_RS12230 2536991..2537599 - 609 WP_044744381.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2532413..2557344 24931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-117)

Antitoxin

SpoVT-AbrB domain

(34-69)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14359.37 Da        Isoelectric Point: 4.6172

>T63072 WP_044744379.1 NZ_CP015436:c2532799-2532413 [Anoxybacillus sp. B7M1]
MPADVPERGDFVVLNFHPQAGHEQAGRRNAIVLSPKEFNQATGFIVVCPMTNQKKGYPFEVDLPKEGISLDGDGYPITGV
VLTDQIKSLDWKARNLTILKKYNPEDAQIEEIDRIIDECLAKIETYLT

Download         Length: 387 bp

>T63072 NZ_CP015436:c2532799-2532413 [Anoxybacillus sp. B7M1]
ATGCCAGCAGACGTACCAGAGAGAGGCGATTTCGTTGTTTTAAACTTCCATCCACAAGCTGGTCATGAGCAGGCCGGGAG
AAGGAATGCTATTGTTTTATCGCCTAAAGAATTCAATCAAGCAACAGGATTTATCGTTGTTTGTCCGATGACCAATCAAA
AGAAGGGTTATCCCTTCGAAGTAGATTTACCAAAAGAAGGAATATCCCTTGATGGCGATGGCTATCCAATAACAGGGGTA
GTTTTAACGGATCAAATTAAATCATTGGATTGGAAAGCTCGTAATCTAACAATTTTAAAGAAGTATAATCCAGAAGATGC
ACAAATTGAAGAAATAGATAGAATCATTGATGAATGCCTAGCGAAAATAGAAACATATCTGACTTGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11492.10 Da        Isoelectric Point: 6.4776

>AT63072 WP_044744380.1 NZ_CP015436:c2533107-2532799 [Anoxybacillus sp. B7M1]
MNAKEHLESKINQSKGGDYMSTVVAQKWGNSLGIRIPKDVADQIGIKQGSEMELNVIGNEGIITLKPKRTRKKYTLEELL
SQITPENRHEEIDFGVEGRELI

Download         Length: 309 bp

>AT63072 NZ_CP015436:c2533107-2532799 [Anoxybacillus sp. B7M1]
ATGAACGCGAAGGAACATTTAGAAAGCAAAATCAACCAAAGTAAAGGAGGGGACTACATGTCTACAGTTGTTGCTCAAAA
ATGGGGAAACAGTTTGGGCATTCGTATCCCAAAAGATGTTGCTGATCAAATAGGGATCAAACAAGGTTCCGAAATGGAAT
TAAACGTGATTGGAAATGAAGGGATCATCACATTAAAGCCAAAAAGAACACGGAAGAAATACACATTGGAAGAACTGCTA
TCACAGATTACACCTGAAAATCGGCACGAGGAAATTGATTTTGGGGTAGAAGGGCGTGAATTGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A160FBF3

Antitoxin

Source ID Structure
AlphaFold DB A0A160FBE5

References