Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2926729..2927423 Replicon chromosome
Accession NZ_CP015435
Organism Anoxybacillus sp. B2M1

Toxin (Protein)


Gene name mazF Uniprot ID A0A160FBF3
Locus tag GFC28_RS14220 Protein ID WP_044744379.1
Coordinates 2927037..2927423 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A160FBE5
Locus tag GFC28_RS14215 Protein ID WP_044744380.1
Coordinates 2926729..2927037 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFC28_RS14190 2922237..2922845 + 609 WP_044744381.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein -
GFC28_RS14195 2922845..2923330 + 486 Protein_2761 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
GFC28_RS18965 2923895..2924110 + 216 WP_156487540.1 ATP-binding protein -
GFC28_RS19535 2924540..2924909 - 370 Protein_2763 hypothetical protein -
GFC28_RS18975 2924996..2925088 + 93 Protein_2764 zinc-ribbon domain-containing protein -
GFC28_RS18980 2925636..2926229 + 594 Protein_2765 pre-toxin TG domain-containing protein -
GFC28_RS14215 2926729..2927037 + 309 WP_044744380.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GFC28_RS14220 2927037..2927423 + 387 WP_044744379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GFC28_RS14225 2927466..2927801 + 336 Protein_2768 hypothetical protein -
GFC28_RS14230 2927798..2928120 + 323 Protein_2769 hypothetical protein -
GFC28_RS19540 2928210..2928431 - 222 Protein_2770 transposase -
GFC28_RS14235 2928490..2929104 - 615 WP_172798625.1 hypothetical protein -
GFC28_RS14240 2929198..2929836 - 639 WP_044744377.1 Bax inhibitor-1/YccA family protein -
GFC28_RS14245 2929944..2930300 + 357 WP_044744376.1 hypothetical protein -
GFC28_RS14250 2930293..2931783 - 1491 WP_044744375.1 glycerol kinase GlpK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2902492..2927423 24931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-117)

Antitoxin

SpoVT-AbrB domain

(34-69)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14359.37 Da        Isoelectric Point: 4.6172

>T63070 WP_044744379.1 NZ_CP015435:2927037-2927423 [Anoxybacillus sp. B2M1]
MPADVPERGDFVVLNFHPQAGHEQAGRRNAIVLSPKEFNQATGFIVVCPMTNQKKGYPFEVDLPKEGISLDGDGYPITGV
VLTDQIKSLDWKARNLTILKKYNPEDAQIEEIDRIIDECLAKIETYLT

Download         Length: 387 bp

>T63070 NZ_CP015435:2927037-2927423 [Anoxybacillus sp. B2M1]
ATGCCAGCAGACGTACCAGAGAGAGGCGATTTCGTTGTTTTAAACTTCCATCCACAAGCTGGTCATGAGCAGGCCGGGAG
AAGGAATGCTATTGTTTTATCGCCTAAAGAATTCAATCAAGCAACAGGATTTATCGTTGTTTGTCCGATGACCAATCAAA
AGAAGGGTTATCCCTTCGAAGTAGATTTACCAAAAGAAGGAATATCCCTTGATGGCGATGGCTATCCAATAACAGGGGTA
GTTTTAACGGATCAAATTAAATCATTGGATTGGAAAGCTCGTAATCTAACAATTTTAAAGAAGTATAATCCAGAAGATGC
ACAAATTGAAGAAATAGATAGAATCATTGATGAATGCCTAGCGAAAATAGAAACATATCTGACTTGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11492.10 Da        Isoelectric Point: 6.4776

>AT63070 WP_044744380.1 NZ_CP015435:2926729-2927037 [Anoxybacillus sp. B2M1]
MNAKEHLESKINQSKGGDYMSTVVAQKWGNSLGIRIPKDVADQIGIKQGSEMELNVIGNEGIITLKPKRTRKKYTLEELL
SQITPENRHEEIDFGVEGRELI

Download         Length: 309 bp

>AT63070 NZ_CP015435:2926729-2927037 [Anoxybacillus sp. B2M1]
ATGAACGCGAAGGAACATTTAGAAAGCAAAATCAACCAAAGTAAAGGAGGGGACTACATGTCTACAGTTGTTGCTCAAAA
ATGGGGAAACAGTTTGGGCATTCGTATCCCAAAAGATGTTGCTGATCAAATAGGGATCAAACAAGGTTCCGAAATGGAAT
TAAACGTGATTGGAAATGAAGGGATCATCACATTAAAGCCAAAAAGAACACGGAAGAAATACACATTGGAAGAACTGCTA
TCACAGATTACACCTGAAAATCGGCACGAGGAAATTGATTTTGGGGTAGAAGGGCGTGAATTGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A160FBF3

Antitoxin

Source ID Structure
AlphaFold DB A0A160FBE5

References