Detailed information of TA system TA063068

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 265683..266490
• Replicon: chromosome
• Accession: NZ_CP015435
• Organism: Anoxybacillus sp. B2M1

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: -
• Locus tag: GFC28_RS01420
• Protein ID: WP_044741982.1
• Coordinates: 265683..266093 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: GFC28_RS01425
• Protein ID: WP_156487495.1
• Coordinates: 266083..266490 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFC28_RS01410	261885..263213	+	1329	WP_044741980.1	ATP-binding protein	-
GFC28_RS01415	263216..265354	+	2139	WP_044741981.1	DEAD/DEAH box helicase	-
GFC28_RS18585	265491..265661	+	171	Protein_272	transposase	-
GFC28_RS01420	265683..266093	-	411	WP_044741982.1	DUF86 domain-containing protein	Toxin
GFC28_RS01425	266083..266490	-	408	WP_156487495.1	nucleotidyltransferase domain-containing protein	Antitoxin
GFC28_RS01430	266760..267776	+	1017	WP_044741984.1	hypothetical protein	-
GFC28_RS01435	267764..269263	+	1500	WP_052660919.1	hypothetical protein	-
GFC28_RS01440	269260..270006	+	747	WP_044741986.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	259311..276847	17536

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (14-131)

Antitoxin

• Polymerase beta, nucleotidyltransferase (9-99)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15889.42 Da
• Isoelectric Point: 7.0499

>T63068 WP_044741982.1 NZ_CP015435:c266093-265683 [Anoxybacillus sp. B2M1]
MKNDVILNKISVIERCIKRIHEEYENNPKNLENYTKQDSMILNLQRACEASIDLAMHMVAQKKLGLPQNSRDAFSLLEQH
GIITPFLSQKMKAMVGFRNIAVHDYQEINLTILQKILDHHLTDFTEFTKAILFYEQ

Nucleotide

• Length: 411 bp

>T63068 NZ_CP015435:c266093-265683 [Anoxybacillus sp. B2M1]
ATGAAAAATGATGTCATTTTAAATAAAATTAGCGTGATCGAGCGGTGCATAAAGCGCATTCACGAAGAATATGAGAACAA
CCCGAAAAATTTAGAAAACTACACGAAGCAAGACTCCATGATCTTAAATCTCCAACGAGCATGTGAAGCAAGCATCGACC
TGGCGATGCACATGGTCGCACAGAAAAAGCTAGGCCTCCCCCAAAACAGTCGAGACGCTTTTTCTCTTTTAGAACAGCAC
GGCATCATCACTCCTTTTCTTTCACAAAAAATGAAAGCGATGGTCGGATTTAGAAACATCGCTGTTCACGACTACCAGGA
GATTAATTTGACAATTTTGCAAAAAATACTTGATCACCATCTAACCGACTTTACCGAGTTCACGAAAGCGATTCTTTTTT
ATGAACAGTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15629.69 Da
• Isoelectric Point: 4.6889

>AT63068 WP_156487495.1 NZ_CP015435:c266490-266083 [Anoxybacillus sp. B2M1]
MTEQIEPIIIEVLTETVSPYLIYLFGSTVTGHVHQDSDIDIAFLSDQKQLDHYERFMVAQELASKLHRDVDLVDLKQAST
VFQAQVVSNGKVIYCTNEQKKAEYEWRALKMYAKLNEERTEILRNINESGSVYEK

Nucleotide

• Length: 408 bp

>AT63068 NZ_CP015435:c266490-266083 [Anoxybacillus sp. B2M1]
TTGACGGAACAGATCGAGCCAATCATTATTGAAGTGTTGACCGAAACGGTGTCCCCATACTTGATTTATTTGTTTGGATC
AACGGTTACAGGACATGTTCATCAAGACAGCGACATTGATATCGCTTTTCTCAGCGACCAAAAACAATTGGATCATTATG
AACGATTTATGGTTGCGCAAGAATTGGCATCCAAACTGCACCGGGACGTCGATCTTGTGGACCTCAAACAAGCAAGCACT
GTGTTTCAAGCACAAGTGGTCAGTAATGGCAAAGTCATCTATTGTACAAATGAACAGAAAAAAGCCGAATATGAATGGAG
AGCCTTGAAGATGTACGCAAAATTGAATGAAGAACGCACGGAAATCTTGCGAAACATTAATGAAAGCGGGTCGGTTTATG
AAAAATGA