Detailed information of TA system TA063067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 4287623..4288231
• Replicon: chromosome
• Accession: NZ_CP015423
• Organism: Paenibacillus polymyxa strain J

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: AOU00_RS19190
• Protein ID: WP_007429335.1
• Coordinates: 4287881..4288231 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AOU00_RS19185
• Protein ID: WP_170970806.1
• Coordinates: 4287623..4287874 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOU00_RS19170	4283001..4284387	-	1387	Protein_3756	helix-turn-helix domain-containing protein	-
AOU00_RS19175	4284568..4285845	+	1278	WP_069291372.1	outer membrane lipoprotein carrier protein LolA	-
AOU00_RS19180	4286103..4287299	+	1197	WP_069291373.1	alanine racemase	-
AOU00_RS19185	4287623..4287874	+	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
AOU00_RS19190	4287881..4288231	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AOU00_RS19195	4288411..4290633	+	2223	WP_069291374.1	RNA-binding transcriptional accessory protein	-
AOU00_RS26350	4290739..4290873	-	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
AOU00_RS19200	4291018..4291542	+	525	WP_069291375.1	SprT family protein	-
AOU00_RS19215	4291930..4292805	-	876	WP_069291376.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T63067 WP_007429335.1 NZ_CP015423:4287881-4288231 [Paenibacillus polymyxa]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T63067 NZ_CP015423:4287881-4288231 [Paenibacillus polymyxa]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGTCCTACTGTGATCGTAGCAGCCATCACCGCCCAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCCGTTGTTCTTCTGGAGCAAATT
CGGACGATCGACAAGCAGAGGCTTACCGACAAAATCACCCATTTAGACGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
ACAAATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT63067 WP_170970806.1 NZ_CP015423:4287623-4287874 [Paenibacillus polymyxa]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT63067 NZ_CP015423:4287623-4287874 [Paenibacillus polymyxa]
ATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGCTATGGAAAATTCCAACCGCAGTGAATTTAT
CAGGCAGGCCATGAAGCTGTATGTAGGTGAACGAAAAAAACGTTACATTCGTGAAGCTATGCAGCGTGGATACATGGAAA
TGGCTAAAATTAACTTAACCATGGCATCCGAAGCCTTTCACGCGGAGGAAGATGCAAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1